The Effect Of Crosslinking With Genipin,Glutaraldehyde Or EDC/NHS On Constructing Collagen/Chitosan Dermal Scaffolds

Cross-linking is one of the most effective ways to improve the mechanical properties of collagen/chitosan dermal scaffolds, to find the optimal cross-linking solution is the focus of research in recent years. In this paper, the most popular cross-linking agents, glutaraldehyde, EDC/NHS and Genipin was utilized to cross-link collagen/chitosan dermal scaffolds in equal molar concentration by one-step cross-linking process. The materials science and biological properties of scaffolds to promote the dermal regeneration was evaluated by in vitro assessment and in vivo transplantation studies, with a view to find the best crosslinking method of the collagen/chitosan dermal scaffolds in the further application. The study results showed that the mechanical properties of the scaffolds cross-linked with genipin was similar with those cross-linked with glutaraldehyde. much better than those cross-linked with EDC/NHS. The biocompatibility of the scaffolds cross-linked with genipin was similar with those cross-linked with EDC/NHS. much better than those cross-linked with glutaraldehyde. The dermal scaffolds crosslinked with genipin had good resistance to wound contraction. The dermal scaffolds crosslinked with genipin mediated dermal regeneration process three-dimensionally with a very clear hierarchy, orderly and controlled mechanism. Genipin-crosslinked scaffolds promoted vascularization better.Research results show that genipin is a potential collagen/chitosan dermal scaffold crosslinking agent, genipin in appropriate concentration was recommended as the crosslinking agent for collagen/chitosan dermal scaffolds.