The Role Of Deslanoside In Gastric Cancer Cells

Objective:To investigate the inhibiting effects of deslanosideon anti-proliferation and apoptosis-induced of gastric cancer cell linesSGC7901and MKN28, and to explore the anticancer mechanism of deslanosideand also to provide experimentalevidence and the theoretical basis for clinical application.Methods:The morphologicalchanges of gastric cancer cells were observed by inverted microscope. The inhibitory effect on cell proliferation was assessed by MTS assay. The cell cycle and apoptosisof gastric cancer cells were detected with Flow Cytometry(FCM). Western blot was applied to detect the changes of relevant protein level. Plasmid was used as the vectorfor the overexpression of ATP1B1gene in gastric cancer cells. The siRNAtransfection was adopted for the knockdown of ATP1B1.All the statistical analysis was performed in the package of SPSS20.0,using varianceanalysis, with a statistically significant level "α=0.05"Results:①With the inverted microscope, we observed the morphological changes of apoptotic gastric cancer cells in deslanosidegroups;②Deslanoside could inhibit gastric cancer cell proliferation andinduce apoptosis in a time-dose dependent manner(p<0.05); ③The cell cycle of gastric cancer cells was detected with FlowCytometry(FCM). When deslanosidegroups were compared with the control group, the distribution of cell cycle in deslanosidegroup changed significantly(p<0.05). The blocking of the cell cycle was in G2/M-phase;④The protein levels were detected with Western blot.When deslanoside groups were compared with the control group, ATP1B1protein decreased in a time-dose dependent manner. While the ERS and apoptosis relevant proteins changed according to that of ATP1B1.⑤ATP1B1silence alone could induce ERS. What’s more, the overexpression of ATP1B1could rescue the ERS induced by deslanoside.Conclusion:①Deslanosidecould significantly inhibit gastric cancer cell proliferation and induce apoptosis in a time-dose dependent manner;②Deslanoside could affect the distribution of gastric cancer cell cycle, inducing G2/M-phase arrest.③Deslanoside could induce ERS by down-regulating the level of ATP1B1, resulting in cell apoptosis.