| Fatal mushroom poisoning incidents annually occurs over the world. The peptide toxins contained in some Amanita species are mainly responsible for the mortality. But the effective drugs for the treatment of Amanita poisoning is still lack. Previous research has shown that Ganoderma lucidwn has very good therapeutic effect for the Amanita poisoning. This paper intends to extract the active constituents of G lucidum---he total triterpenoids and its hepatoprotective effects on a-amanitin induced Liver Injury in mice and its antioxidant mechanism, the results were as follows:1. The extraction and the purification of total triterpenoid from G lucidum were as follows:In this experiment, the supercritical carbon dioxide extraction method was used to extract the objective product from the powder of G lucidum fruiting body. The95%ethanol was acted as the cosolvent. The conditions of extraction were as follows:The temperature was40℃; the pressure was35Mpa; the CO2flow rate was35g/min and the volume/weight ratio of cosolvent and powder was6:1(mL/g). The supercritical extraction rate was2.42%. Then extracts from supercritical were further separated by silica gel column chromatography, and the mobile phase was the different proportions of chloroform and methanol. The objective product was mainly concentrated in the first two mobile phases of elution ratio.2. Preliminary experiments of hepatoprotection of G lucidum total triterpenoids (GLTT) for hepatic injury induced by a-amanitin poisoning:The normal control group, a-amanitin poisoning group, discretion dose GLTT group, silybin and pure olive oil group were setted. The results showed that the various biochemical indicators in a-amanitin poisoning group were significantly different from the normal control group. The high dose (200mg/kg/day) and the low dose (50mg/kg/day) GLTT as experimental group were selected randomly. The GLTT of two groups was dissolved in olive oil. According to the results, we found that the GLTT groups showed a certain efficacy. Besides, the various biochemical indexes in the olive oil group had no obvious difference with the poisoning group, so the possible impact of olive oil can be omitted.3. The hepatoprotection of different doses GLTT for hepatic injury induced by a-amanitin were analyzed. The normal control group, a-amanitin poisoning group, olive oil group and different doses GLTT group (Dose of25,50,100,200,400mg/kg/day, lavage treatment) were setted. The results indicated that the hepatoprotection effect with GLTT was dose-related in a certain range. With the dose increased (20mg/kg/day to200mg/kg/day), all indexes in the serum and the liver homogenate were close to the normal group. When the dose increased to100mg/kg/day and200mg/kg/day, the serum transaminase activity and the indicators in liver homogenate which were compared in the poisoning group had improved significantly.4. The different delivery times with GLTT administration for the a-amanitin-induced liver injury were studied. The normal control group, a-amanitin poisoning group and different delivery time GLTT group (After the injection of toxins in Oh,1h,2h,3h,4h,5h respectively) were setted. The results showed that the earier delivery time of GLTT could make the less liver injury, The enzyme indexes in serum and the biochemical indicators in liver homogenate were closer to the level of the normal group. The results told us that it is necessary to take a treat as soon as possible after the mushroom poisoning.5. The mortality of mice with the GLTT administration group, the silybin administration group, the a-amanitin group and the normal control group was statisticed. After5day’s administration, there was no death in the normal group, but the mortality reached to96.67%in the α-amanitin poisoning group, while the mortality rate were46.67%and56.67%respectively in the GLTT administration group and the silybin group. So we thought that the GLTT had distinct effect on the detoxification in the a-amanitin poisoning.Above all, the GLTT could significantly reduce liver injury in mice which were caused by a-amanitin, thus we can used it as a medicine choice for the a-amanitin poisoning. |
PDF Full Text Request