The Molecular Mechanism Of Dendrobium Compound To Promote Islet Cell Proliferation By GLP-1/β-Catenin Signaling Pathway

ObjectiveTo investigate Dendrobium Compound’s effect on GLP-1R->cAMP->PKA-> P-B-Catenin(Ser675)->PDX-1pathway, pancreatic B cell proliferation, insulin secretion and insulin gene expression in order to lay a theoretical clinical basis for diabetes mellitus treatment.MethodsCells were cultivated and randomly divided into normal control group, the inhibitor group, Dendrobium Compound group and Exendin-4positive control group, After24hours, cells were collected with cell cycle measured by flow cytometry, insulin secretion of cell supernatant investigated by radioimmunoassay, intracellular cAMP level tested by enzyme-linked immunosorbent, gene expression of GLP-1R and PDX-1quantified by Q-PCR and protein expression of B-Catenin, P-B-catenin (Ser-675), GLP-1R and PDX-1measured by western blotting.Results1. Compared with the control group, Exendin9-39treatment downregulated the S phase, DC and Exendin-4treatment upregulated the S phase.2. Compared with the control group, Exendin9-39treatment significantly downregulated the levels of insulin and cAMP, DC and Exendin-4treatment upregulated the levels of insulin and cAMP.3. Compared with the control group, Exendin9-39treatment downregulated the expression levels of GLP-1R, PDX-1mRNA. DC and Exendin-4treatment significantly upregulated the expression levels of GLP-1R, PDX-1mRNA.4. Compared with the normal group, Exendin9-39treatment downregulated the expression levels of GLP-1R,(3-Catenin, P-B-catenin (Ser-675), PDX-1protein, DC and Exendin-4treatment significantly upregulated the expression levels of GLP-1R, B-Catenin, P-P-catenin (Ser-675), PDX-1protein.ConclusionDendrobium Compound stimulated insulin gene expression and promoted islet cell proliferation via GLP-1R->cAMP->PKA->P-B-Catenin (Ser675)-> PDX-1signaling pathway.