| Objective: Ovarian cancer (ovary cancer, OC) is one of the mostcommon female genital tract malignancy,accounting for sixth place in allfemale cancers.Epithelial ovarian cancer (epithelial ovary cancer EOC) is themost common ovarian cancer, accounting for about50%-70%of primaryovarian tumors.The highest fatality rate of all gynecological tumors,resultingin approximately125,000patients died each year.Epithelial ovarian cancermortality is high mainly because of its rapid onset and lack of specificity earlysigns and symptoms,more than70percent of ovarian cancer patients newlydiagnosed advanced stage,lost the best timing of treatment.Detect early tumorssuch as ovarian cancer is still confined to the ovary,the5-year survival rate isgreater than90%.Advanced disease caused by adhesions cancer,distantmetastasis so that surgery can not completely remove the lesion.Present,thepreferred treatment options of ovarian cancer is cytoreductive surgery pluschemotherapy,cisplatin and paclitaxel as first-line solution.Although thediagnosis and chemotherapy technology continues to develop,but so far the5-year survival of patients with advanced still only30%,and most patients willrelapse and become resistant.In recent years,with the deepening of cancer research scholars are keenon Cancer stem cell theory.Stem cells refers to a class of cells,that the kind ofundifferentiated,unlimited proliferation and differentiation potential of a classof cells.Signal molecules Octamer4(OCT4) is a member of the POUfamily,highly expressed in cancer stem cells,embryonic stem cells and adultstem cells.OCT4over expression can be maintained embryonic stem cellself-renewal,unlimited proliferation and differentiation capacity.MicroRNAsare a class of small RNA molecules,which can interaction a specific area of the target mRNA,in the way of not completely complementary ofthree,causing the cutting mRNA cleavage or translational inhibition of theprotein,thereby affecting the expression and function of the targetgene.Previous studies have found that OCT4expression in endometrial cancerpatients was negatively correlated with tumor differentiation grade,andmiR-145in contrast with tumor grade was positively correlated.Thus,wespeculate whether the role of miR-145is also available in epithelial ovariancancer in OCT4,by inhibiting the expression of OCT4to have a positiveimpact on the role of drug-resistant and recurrence tumor.This study willobserve the expression of miR-145and OCT4in ovarian cancer tissues withdifferent cancer patients, and analyze its relationship with tumor recurrenceand drug resistance in order to be able to find a sensitive and effectivedetection method for epithelial ovarian cancer prognosis determine theefficacy of analysis and provide a favorable basis for clinical work to solve theexisting problems.Methods: Select6cases of normal ovarian tissue,29cases of epithelialovarian cancer cases from the Second Hospital of Hebei MedicalUniversity,Fourth Hospital of Hebei Medical University Third Hospital in2011-2013,and the first by the Hebei Medical University Hospital pathologyconfirmed verified as epithelial ovarian cancer.According to the experimentalgroup of not recurrent epithelial ovarian cancer and recurrent epithelialovarian cancer and the control group.To detect the relative levels of miR-145by quantitative real-time PCR and the relative levels of OCT4byimmunohistochemistry.Discussion The related research on relapse and drugresistance of epithelial ovarian cancer and the expression levels ofMicroRNA-145and OCT4in tumor tissue.Result:1The relative level of oct4in different tissuesImmunohistochemistry showed,OCT4in normal ovarian tissue,epithelialovarian cancer is not positive expression rate of drug-resistant group and thegroup were20.00%,36.37%,82.35%. 1.1the expression level of OCT4in normal ovarian tissue and resistancegroups of no significant difference.compared with normal ovarian tissue andnot recurrent epithelial ovarian cancer P>0.05(Fisher’s Exact TestP=0.60>0.05)；1.2The expression level of OCT4below the resistance group in the normalgroup, there were significant differences between the two.compared withnormal ovarian tissue and recurrent epithelial ovarian cancer P<0.05(Fisher’sExact Test P=0.009<0.05）；1.3The expression level of OCT4below the resistance group in the notresistance group,there were significant differences between the two.comparedwith not recurrent epithelial ovarian cancer and recurrent epithelial ovariancancer P<0.05(Fisher’s Exact Test P=0.02<0.05).2The relative level of miR-145in different tissuesReal-Time PCR results showed that, MiR-145in normal ovarian tissue,epithelial ovarian cancer is not resistant group and drug group graduallyincreased expression levels were1.0483±0.8989,0.8236±0.6423,0.5224±0.7862.And there were significantly different in the three groups.2.1miR-145in normal ovarian tissue expression level is higher than the notrecurrent group,there were significant differences between the two.comparedwith normal ovarian tissue and not recurrent epithelial ovarian cancerP<0.05(P=0.00<0.05);2.2miR-145in normal ovarian tissue expression level is higher than therecurrent group,there were significant differences between the two.comparedwith normal ovarian tissue and recurrent epithelial ovarian cancerP<0.05(P=0.00<0.05);2.3miR-145in the not recurrent group expression level is higher thanrecurrent group,there were significant differences between the two.comparedWith recurrent epithelial ovarian cancer and not recurrentP<0.05(P=0.00<0.05).Conclusion:In this study,immunohistochemistry and quantitativereal-time PCR method to detect the expression of OCT4and miR-145in different tissues,the results show that:OCT4in normal ovarian tissue andepithelial ovarian carcinoma were expressed, and elevated expression levels incancer tissues.The expression level of OCT4below the resistance group in thenot resistance group,there were significant differences between thetwo.miR-145in normal ovarian tissue and epithelial ovarian cancer tissuesalso were expressed,but its opposite,miR-145levels and OCT4expressiondecreased in cancer tissues.miR-145in the not recurrent group expressionlevel is higher than recurrent group,there were significant differencesbetween the two.But in epithelial ovarian cancer if there is an inherent linkbetween OCT4and miR-145,and whether the OCT4and miR-145can as asensitive detection method for determining the prognosis and efficacy ofepithelial ovarian cancer patients, it needs further research. |
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