Effects Of Oncolytic Adenovirus Combined With Radiation Therapy In Bladder Tumor Cells

Objective To investigate the effect of oncolytic adenovirus (Ads) combined with radiation therapy in bladder tumor cells.Methods The cell biability was determined by CCK-8(cell counting kit-8) assay. Cell revolution and apoptosis were analyzed by flow cytometry. The mRNA expression of Bax, Bcl-2、 BECN、Atg-5、CAR were analyzed by realtime-PCR. The protein level of E1A, Bax, Bcl-2, Cytochromin c were measured by Western blot.Results Oncolytic adenovirus combined with radiation therapy significantly inhibited the proliferation of bladder tumor cells in an concentration-dependent manner, indicating a synergetic effect. Flow cytometry showed that combination treatment could improve the apoptosis rate of bladder tumor cells. The mRNA expression of Bax, CAR was up-regulated and the mRNA expression of Bcl-2was down-regulated (p<0.05). Compared with the contral group, the autophagy related gene BECN、Atg-5mRNA were down-regulated (p<0.05).The protein level of E1A,Bax, Cytochromin c (cytoplasma) were up-regulated and the protein level of Bcl-2, Cytochromin c (mitochomdria) were down-regulated by combination treatment.Conclusion Oncolytic adenovirus combined with radiation therapy exerts its antitumor effect through inducing mitochondrial apoptosis pathway and had a synergetic effect.