| The development of Lung cancer are complicated processes that involve a variety ofabnormal gene regulation and multiple protein expression. As the research of lung cancer withmolecular biology,we can intervene molecular biological targets of lung cancer for earlydetection and early treatment. In recent years, as with research and development of molecularbiology and human genomics, β-catenin and MMP-2role in tumorigenesis is starting to getattention. As a kind of multi-functional protein, β-catenin play an important role in in thecell adhesion and Wnt/β-catenin signal transduction pathways. Abnormal accumulation ofβ-catenin in lung cancer cells has been correlated with disease initiation, progression,invasiveness, and metastasis. When Wnt/β-catenin signal transduction pathways get activated,normal intracellular signal transduction go wrong, phosphorylation and degradation of betacatenin are interrupted, a large number of free beta catenin are accumulated in the cytoplasmand shift into cell nucleus where beta catenin start target gene transcription,which result inabnormal cell proliferation, differentiation, and eventually occurrence of tumor. MMP-2is animportant member of the family of matrix metalloproteinases,and mainly break theextracellular matrix to create favorable conditions for tumor invasion and metastasis. Inaddition, MMP-2can also promote tumor invasion and metastasis by controlling theformation of tumor blood vessels and regulating tumor cells and matrix adhesion. Therefore,through researching beta-catenin and MMP-2expression and observing their relations inlung cancer, we can find new targets for diagnosis and treatment of lung cancer.Objective:At present，their is little research about the expressions of MMP-2and itsrelationship with β-catenin in non-small cell lung carcinoma.In this study，we examined theexpression of β-catenin and MMP-2in non-small cell lung carcinoma byimmunohistochemistry，and conducted correlation analysis combined with clinical date toexplore the value for NSCLC diagnosis and differentiation. Methods:A total of39lung cancer patients received surgery at the Department ofThoracic Surgery in the First Affiliated Hospital of Jilin University between April2012andAugust2012. All patients did not receive preoperative radiation and chemotherapy and othertreatments, and there is a clear postoperative pathological diagnosis. The collection of clinicaldata included geneder,age,pattern of organition,Lymph node metastasis and tumor size.Therewere27cases of male and12case of female with the average age of59years between43and76years.Twenty lung adenocarcinoma and19lung squamous cell carcinoma wereconfirmed histologically.Lymph node metastasis was found in18cases. Tumor size less than3×3×3cm3was found in12cases.Eight adjacent normal-appearing lung tissues were obtainedas controls. We examine the expression of beta-catenin and MMP-2in patients withNSCLC by immunohistochemical method.After collating dyed samples, the comparison ofdifferent clinicopathologic features were calculated using the χ2test and Fisher’s exact test.Grade correspondence analysis was performed using nonpara-metric Spearman rankcorrelation analysis.Result：β-catenin protein was mainly localized in both the cell membrane and cytoplasmthat was brown or yellow staining.Similarly, MMP-2was located in the cell cytoplasm withbrown or yellow staining. Expression of cytosolic β-catenin and MMP-2in NSCLC tissuewas significantly higher than that in normal tissues (P <0.005).β-catenin associated andMMP-2expression in lung cancer was not with patient’s age, gender, or tumor size (P>0.005).cytosolic protein expression of β-catenin in lung squamous cell carcinoma was significantlylower than that that in lung adenocarcinoma (P=0.02). However, cell membrane proteinexpression of β-catenin in squamous cell carcinoma was elevated compared to inadenocarcinoma(P=0.041).3. MMP-2expression in N1–2NSCLC patients was significantlyincreased relative to N0patients (P=0.019).4.statistical analysis showed no correlationbetween β-catenin and MMP-2expression in NSCLC samples.Conclusions：cytosolic protein expression of β-catenin and MMP-2in NSCLC samplesis increased relative to normal lung tissues. Also, expression of β-catenin is significantlylower in squamous cell carcinoma than that in lung adenocarcinoma subtypes. Additionally, MMP-2expression in N1–2NSCLC tissues is higher than that in N0lung tissue. There is nocorrelation between β-catenin and MMP-2expression in NSCLC, but the increase ofMMP-2expression and reduce of β-catenin expression in cell membrane may participate in theprocess of tumor cell transfer together. |
