The Correlation Between STAT3Gene And Thyroid Cancer Research

Background and purpose：Thyroid cancer in the human head and neck malignant tumor is the mostcommon and frequent, and the endocrine system of the most common malignanttumor.Its pathogenesis is not fully clear at present, consideration and radiation,genetic, diet and other factors related.The main treatment includes surgical treatment,131I treatment of thyroid hormone suppression therapy and postoperative.Most ofpapillary carcinoma, follicular carcinoma patients prognosis is good, but someintractable medullary thyroid carcinoma, such as cancer, undifferentiated carcinomaand recurrence of differentiated thyroid cancer treated by surgery,131I, thyroidhormone suppression therapy and rehabilitation treatment means such as hard toachieve good therapeutic effect.With the rapid development of molecular biologyand immunology technology, gene therapy has been used in the diagnosis andtreatment of various diseases, the method is simple, strong pertinence, effective, andin the treatment of patients with pain in the process of small, so the gene therapymay be a clinical doctor new treatment for thyroid cancer especially thyroidcarcinoma refractory, bring a historical breakthrough for the treatment of thyroidcancer.Methods：This study collected clinical thyroid cancer tissue as the research object, and thecorresponding tissue adjacent to carcinoma and normal thyroid tissue contrastanalysis.The first part of application S-P immunohistochemical method is used toanalyze thyroid cancer, carcinoma adjacent tissues and STAT3expression in normalthyroid tissue.The second part using rt-pcr technology detection of carcinoma tissueand branch into normal thyroid tissue in the control group STAT3mRNAabundance;And joint using Western blot technique and IHC testing STAT3proteinand activation form STAT3(pSTAT3) protein expression differences in three groups of specimens and positioning, and detection of STAT3signal transduction pathwaymembers (VEGF, Cyclin D1) expression.Results：1. The expression of STAT3in thyroid carcinoma tissue was obviously higherthan tissue adjacent to carcinoma, STAT3in the expression of tissue adjacent tocarcinoma is higher than normal thyroid tissue.2. The content of STAT3mRNA in carcinoma tissues and adjacent tissues andnormal thyroid tissue existed in the difference (p <0.05). In24cases of thyroidcarcinoma specimens, phosphorylated STAT3pSTAT3protein expression was21cases, accounting for87.5%of the total (21/24),8cases of carcinoma by controlgroup to see the positive expression of the total33.3%(8/24), not a positiveexpression in normal thyroid tissue. Tissue adjacent to carcinoma tissues and the twogroups was significant difference (P <0.05), carcinoma tissue and normal thyroidtissue in the two groups was significant difference (P <0.01), tissue adjacent tocarcinoma and normal thyroid tissue in the two groups is no statistical difference(P>0.05).3.The expression of Cyclin D1and VEGF in carcinoma tissues and adjacenttissues and normal thyroid tissues were significant difference (p <0.01).Conclusion：STAT3gene expressed in thyroid cancer cells abnormal activation, active, anddownstream genes were obviously up-regulated Cyclin D1, VEGF expression incancerous tissue, thus the STAT active gene may be an important factor in thyroidcancer occurrence and development mechanism.So STAT3can be used as thyroidcancer gene diagnosis, treatment, new targets.Especially for the treatment ofrefractory thyroid cancer brings new hope.