Based On The Median Nerve Conduction Velocity Observe The Experimental Study Of Ea-neiguan Point Anti-ppi-induced Myocardial Ischemia In Rabbits

Objective:To determine the dominant topic Neiguan median nerve conduction velocity as quantitative indicators Neiguan acupuncture signal and median nerve conduction velocitv were based on this observation to explore the electrical acupuncture at Neiguan anti-PPI-induced myocardial ischemia in rabbits protective effect in experimental animals by regulating quantitative indicators points to accurately locate acupuncture on animals, experimental animals make acupuncture more convincing data obtained and credibility, clinical experimental basis.Methods:In this study, select1.5-2.5kg healthy adult rabbits, with BL-420S record their biological functional experimental system11ECG, the ECG abnormalities were excluded, the normal rabbits (male and female) were randomly divided into normal group, model group, sham acupuncture group,62.5m/s group,41.67m/s group,31.25m/s group,25m/s.7groups of10. With20%urethane5ml/kg by rabbit ear vein injection of anesthesia. the separation of the median nerve trunk, use the BL-420S system to detect biological and functional experimental nerve fiber conduction velocity. Methods for acute ischemic modeling ear vein injection and continuous infusion of PP1approach. BL-420S biological function using the experimental system, simultaneously recorded the rabbit II ECG HR, PR interval. ST segment, T wave and left ventricular pressure changes LVSP, LVEDP and±dp/dtmax of. ECG recording to the recording electrode needle electrodes penetrate the skin of rabbits limbs, set system parameters:a time constant of0.1s, the amplifier in the range of1mV, scanning speed125ms/div, the filter is50Hz. The inner diameter of the recording made with PT-100biological blood pressure sensor connected to1.5mm when left ventricular pressure polyethylene ventricular cardiac catheterization. the system parameters:experimental pilot projectâ†'cycle testâ†'hemodynamic circulation. Use722grating spectrophotometer using colorimetric determination of serum360min when CK-MB, LDH activity changes ofResults:The results for the normal group10s, lmin,5min,10min,30min, HR60min each time point, PR interval. ST segment. T wave amplitude, LVSP. LVEDP.+dp/dtmax,-dp/dtmax, PPI injection after360min serum LDH, dynamic changes of CK-MB model group10s.1min,5min, lOmin,30min,60min each time point HR, PR interval, ST segment, T wave amplitude,LVSP, LVEDP,+dp/dtmax,-dp/dtmax, when injected360min after PPI serum LDH, CK-MB activity change compared statistically there was a significant difference in the two data sets, P<0.01, illustrated by ear vein injection and continuous infusion PPI method can be successfully replicated in rabbits with acute myocardial ischemia. Sham acupuncture group10s.1min,5min. lOmin,30min,60min each time point HR. PR interval. ST segment, T wave amplitude, LVSP, LVEDP,+dp/dtmax,-dp/dtmax,360min after injection of serum PPI the LDH, CK-MB vitality changes with the model group10s, lmin,5min,10min,30min,60min each time point HR, PR interval. ST segment, T wave amplitude, LVSP. LVEDP.+dp/dtmax,-dp/dtmax, while PPI360min after injection of serum LDH, CK-MB activity comparing changes were statistically no significant difference. P>0.05, sham acupuncture description no protective effect on myocardial ischemia. Treatment group62.50m/s group,31.25m/s group and25.00m/s group10s.1min,5min. lOmin,30min,60min each time point HR. PR interval, ST segment, T wave amplitude, LVSP, LVEDP,+dp/dtmax,-dp/dtmax, when injected360min after PPI serum LDH, CK-MB of dynamic changes in the model group10s,1min,5min,1Omin,30min,60min each time point HR, PR interval, ST segment, T wave amplitude, LVSP, LVEDP,+dp/dtmax,-dp/dtmax, when injected360min after PPI serum LDH, CK-MB activity change comparison, no significant differences were statistically, P>0.05, description needle thorn Neiguan nerve fiber conduction velocity of62.50m/s,31.25m/s,25.00m/s for the three types of nerve fibers excited myocardial ischemia and no significant protective effect. Treatment group41.67m/s group,1min,5min,10min,30min,60min each time point HR, PR interval, ST segment, T wave amplitude, LVSP, LVEDP,+dp/dtmax,-dp/dtmax, PPI after the injection time360min serum LDH, CK-MB vitality changes with the model group10s,1min,5min, lOmin,30min.60min each time point HR. PR interval. ST segment, T wave amplitude. LVSP. LVEDP.+dp/dtmax. when360min serum LDH after-dp/dtmax, PPI injection, dynamic changes in CK-MB comparison, there are significant differences in the statistical treatment, P<0.05, explain acupuncture Neiguan nerve fiber conduction velocity of41.67m/s nerve fibers excited about myocardial ischemia has a significant protective effect.Conclusion:1. Ear vein injection and continuous infusion of PPI can be successfully replicated in rabbits with acute myocardial ischemia.2. Electro Neiguan, when the median nerve recorded41.67m/s fiber conduction velocity, myocardial ischemia has a significant protective effect.3. Electro Neiguan, when the median nerve to nerve fibers recorded conduction velocity was62.50m/s,31.25m/s.25.00m/s. myocardial ischemia and no significant protective effect.