| Objective: This study through high sugar, high fat feed plus low-doseSTZ method to establish diabetic nephropathy (DN) rats model, and applied todrain turbidity detoxify, invigorate the circulation of t2dm with interventionaltreatment was carried out on the model, through the observation of ratquantitative hutp (24)24hours urinary protein, serum albumin (TP), albumin(propagated),glycosylated hemoglobin (HbA1c),cholesterol (TC), triglycerides(), serum creatinine (Cr), urea nitrogen (BUN), and other biochemicalindicators and kidney tissues Nephrin mRNA expression, discusses on thedrain turbidity detoxify, invigorate the circulation of t2dm party thetherapeutic effect of diabetic nephropathy and its possible mechanism, so as toprovide experimental basis for clinical treatment of diabetic nephropathy andtheoretical basis.Methods: choose85grade clean healthy male SD rats, ordinary adaptivefeed feed for a week, urinary protein quantitative measurement and urinesugar were negative, and then randomly take out15for normal control group,the other70give high sugar and high fat feed. Intraperitoneal injectionSTZ35mg is administered after12w/kg, the normal group was given thecorresponding volume of intraperitoneal injection of citrate buffer.72h afterfasting blood glucose, blood sugar or greater tendency for16.7mmol/Lbuilding success as a diabetes. Building successful rats randomly divided intofour groups by weight, respectively: the model group (MG), Chinese medicinetreatment group (ZYG) and western medicine treatment group (XYG),Chinese medicine and western medicine treatment group (JYG), in addition tothe model group, the treatment group were given corresponding drug lavage,1times a day, the normal group and model group to the corresponding normalsaline, lavage1times a day, a total of16weeks. In4weeks after the treatment (4w),8w,12w at the end of the rat caudal vein blood glucose measurement,give us24hours urine protein urine dipstick test urine protein.16weeks aftertreatment, give us24hours urine, record urine output, centrifugal supernatanton urine take retention for24hours urinary protein quantitative determination,the use of chloral hydrate anesthesia in rats, the abdominal aorta in blood,centrifugal in serum of rats, the measurement of rat serum TP, propagated, Cr,BUN, serum TG, TC and other biochemical indicators, gather the right kidneyand weight, take kidney tissue used in light microscope, electron microscope,immunohistochemical observation and Real-time PCR.Results:1The comparison of experimental rats in generalNormal rats normal diet, urine normal, muscle plump, hair luster,responsive. Module of rats in the process of giving death four, including twodied of model group, traditional Chinese medicine and western medicinegroup each one death, estimation of elevated blood sugar, died of ketoacidosis,the rest were significantly more drinks, food, polyuria, thin body, hair, slow,etc. The sharpest symptoms of model group. In the process of drug delivery,death four model group, Chinese medicine group1only death, only2bloodsugar self-healing, western medicine group2, only1only blood sugarself-healing, traditional Chinese medicine and western medicine combinedtreatment group1only blood sugar self-healing.2Each group in the rat body weight, heavy, and the right kidneyhypertrophy index (=right kidney hypertrophy index weight/body weight)Model group and treatment group compared with normal group were lostweight (P<0.05); Compared with indifference between model group andtreatment group (P>0.05). The normal group, model group, treatment groupcompared with ChongWu right kidney of rats significantly difference (P>0.05). Model group and treatment group of hypertrophy index compared withnormal group were increased (P<0.05); There was no difference in thetreatment group compared with model group (P>0.05).3Comparison of24-hour urine protein Compared with NG, the treatment group and MG significantlyincreased (P<0.05); Compared with the MG, the treatment group significantlydecreased (P<0.05); Compared with traditional Chinese medicine+westernmedicine group, Chinese medicine, western medicine group increased(P<0.05);Compared to the traditional Chinese medicine and western medicinegroup no significant statistical significance (P>0.05).4Each group of glycosy lated hemoglobinCompared with the NG, the hemoglobin a1c in the treatment group andmodel group were increased (P<0.05); Compared with traditional Chinesemedicine+western medicine group, Chinese medicine, western medicinegroup and model group were higher (P<0.05); The traditional Chinesemedicine and traditional Chinese medicine+western medicine group comparedwith no significant difference (P>0.05); Western medicine group and MG hadno significanl significance (P>0.05).5The biochemical indicators of comparisonCompared with NG the TP and ALB of each treatment group and MGwere lower (P<0.05), and TG and TC, increased significantly(P<0.05)); TheTP and ALB of each treatment group, propagated with the model groupincreased significantly (P<0.05), and TG, TC, significantly decreased(P<0.05);Compared with traditional Chinese medicine+western medicine group,Chinese medicine and western medicine group of TP, propagated lower(P<0.05), TG, TC, elevated (P<0.05); Compared to the traditional Chinesemedicine and western medicine group TP, propagated, TG, TC has no obviousstatistical significance (P<0.05).The NG, MG, the treatment group of Scr, BUN compared with nosignificant difference(P<0.05).6Kidney tissue pathological morphology observation6.1then observations show that:NG rat glomerular structure complete, did not see glomerularhypertrophy, glomerular capillary basement membrane thickening not seen,mesangial cells and their matrix has no obvious change. MG visible rat glomerular hypertrophy, glomerular capillary basement membrane thickeningobviously, mesangial area widened. Each treatment group glomerular all havedifferent degree of pathological changes.6.2Under the electron microscope observation showed:Normal group of glomerular basement membrane evenly withoutthickening, foot processes uniform distribution, the model group glomerularcapillary basement membrane thickening, visible part hyperplasia, footprocess fusion widely. Each treatment group of capillary basement membranethickening, different level part foot process fusion.6.3Immunohistochemical staining method to detect the expression ofrenal tissue Nephrin:Nephrin in the NG, the treatment group were expressed, glomerularpodocytes in membrane, express parts of model group is not obvious.Compared with NG, each module of the Nephrin expression decreasedsignificantly(P<0.05); Compared with MG, the express strong Nephrin intreatment group (P <0.05); Between the treatment group compared withstrongest expression of Traditional Chinese medicine+western medicinegroup(P<0.05); Traditional Chinese medicine and western medicine groupwere expressed, compared two groups have no obvious statisticalsignificance(P<0.05).7In the kidney tissues Nephrin mRNA expressionStatistical analysis results show that edicine and western medicine,Chinese medicine, western medicine group in the rat kidney Nephrin mRNAexpression decreased (P<0.05); Compared with model group, traditionalChinese medicine and western medicine group, Chinese medicine, westernmedicine group in the rat kidney Nephrin mRNA expression level (P<0.05);Compared with the traditional Chinese medicine and western medicine group,Chinese medicine and western medicine group Nephrin in the organization ofthe mRNA expression of cut (P<0.05); Traditional Chinese medicine, westernmedicine group (P>0.05) in comparison with no statistical significance. Conclusion1Xiezhuo Jieduo Huoxue Tongluo decoction can invigorate thecirculation of t2dm with Chinese traditional medicine can reduce diabeticnephropathy rats,24-hour urinary protein quantitative increase serum of TP,propagated, reducing TG, Chol, reduce glycated hemoglobin, kidneypathological damage in the rat model of diabetic nephropathy and delay theprogress of diabetic nephropathy.2Xiezhuo Jieduo Huoxue Tongluo decoction can invigorate thecirculation of t2dm can increase the expression of Nephrin, protect glomerularpodocyte, delay the progress of diabetic nephropathy.3In conclusion,combined traditional Chinese medicine+westernmedicine treatment of diabetic nephronpathy effect is better. |
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