Screening The Phamacodynamic Fraction Of Jiaweihuoxiangzhengqi Soft Capsules And Study On The Quality Control

The paper is include four chapters, the first chapter is literature review, the second chapter includes efficacy soft capsules Jiaweihuoxiangzhengqi site screening studies, the third chapter constituents of Jiaweihuoxiangzhengqi soft phamacodynamic fraction capsules effects Chapter four Study Juoxiangzhengqi soft capsules quality standardsLiterature reviewThis paper reviewed more than60documents, including the analysis of Huoxiang Zhengqi powder prescription, finishing chemical constituents of eleven traditional Chinese medicines and pharmacological Jiawei Huoxiang Zhengqi soft capsule on study; and the pharmacodynamics part of research methods were summarized.Chapter I Screening the phamacodynamic fraction of Jiaweihuoxiangzhengqi Soft capsulesThis part first prepared Huoxiangzhengqi Soft capsules class preferred flavore petroleum ether, ethyl acetate, chloroform, butanol water five parts, with three pharmacological models (mouse model of spontaneous contraction of intestinal smooth muscle, anti-inflammatory macrophages in vitro model, TNBS-induced mouse model of inflammatory bowel disease) and five of the original parts of the formulation efficacy screening experiments, the results show that the original formulation, petroleum ether in mice significantly inhibited intestinal contraction, the original formulation, petroleum ether and ethyl acetate extract has significant anti-inflammatory activity of the role models of inflammatory bowel disease, and5parts of the original formulation of TNBS-induced mice have effect. Taken together, select petroleum ether and ethyl acetate parts of constituents of the next.Chapter II Study of the chemical constituents in the phamacodynamic fraction through GC-MS,LC-MS.This chapter using GC-MS analysis of the chemical composition of petroleum ether, the14compounds identified were2-methyl-3-amyl ketone, methyl cyclohexane, palmitoleic acid, ethyl formate,(3-eudesmol, a-eudesmol, patchouli alcohol,1-(2,4,6-hydroxyphenyl) ethyl ketone, atractylenoide III,atractylenoide II,honokiol, magnolol2-[4-Methyl-6-(2,6,6-trimethylcyclo hex-en-1-enyl)hexa-1,3,5-trienyl]cyclohex-1-en-1-carboxalde hyde) l,4-Hexadien-3-one,5-methyl-l-[2,6,6-trimethly-2,4-cyclohexadien-l-yl]-.Ethyl acetate extract using LC-MS analysis of its components.Chapter Ⅲ Study Juoxiangzhengqi soft capsules quality standardsAnalysis is based on the results of the second chapter of the efficacy trials and composition of Chapter III, compared to the2010edition of Chinese Pharmacopoeia Huoxiangzhengqi flavored soft capsules for quality control requirements, measured in terms of measuring and containing fingerprints do to improve. With measured determination:the establishment of a flavored soft capsules Huoxiangzhengqi glycyrrhizin, hesperidin, honokiol and honokiol HPLC method for simultaneous determination; fingerprint:Create flavored soft capsules Huoxiangzhengqi Gas fingerprints on volatile components to product quality control.