Quality Evaluation And Relevant Pharmacodynamics Of Camellia Oleifera

camellia fruit, main object of the thesis, study the biological and economic characters of the different clones and the content of the main chemical composition and antilipemic effect of tea oil and antitumor effect of the total saponins were studied. This thesis mainly consists of six parts, each part of the main content is as follows:1.Through the comprehensive selection of the easy determination of the biological characters, judge and predict quality properties such as yield and quality of tea oil. Through the investigation of the champion trees about the properties.the differential relationship between biological and economic characters is studied by canonical correlation. Weight of per seed and weight of simple fruit are respectively the first and second main factors among the canoical variables, that are significantly related to the content of oleic acid, linoleic acid and oil production of single plant. Canonical Correlation coefficient is0.9494. which reach significant level; the first pair of canonical correlation structure into a comprehensive selection index, makes a comprehensive evaluation of20strains clone C.oleifera and divid into three categories. This method is preliminarily reveal the relationship between characters, provide the reference to selective breeding for camellia oleifera.2. To optimize the extraction technology of total flavonoids from Camellia Seed. Extraction temperature, extraction time, ethanol concentration, solid-liquid ratio as influencing factors, Box-Behnken central composite design was used to establish mathematical model, response surface and contour were made with extract ratio of flavonoids as response value. Optimum extraction technology conditions were extraction temperature66℃, ethanol concentration70%, extracting time1.3.h, solid-liquid ratio of1:22. Under these conditions, yield of flavones from Camellia Seed was1.87%, Close to the theoretical value1.89%. This optimized technology was stable and reliable.3. To establish the relationship between the active component content of active ingredients in kernel of C. oleifera. in order to predict the content of active ingredients. The content of active ingredients was determinated by Ultraviolet spectrophotometer. correlation analysis methods was used to analyze the correlation between components, establish the curve regression model. The total saponins and flavonoids. total polyphenol and total protein is significantly positive correlation, regression equation were Y=6.076+10.698X+8.824X2+3.871X3, Y=0.045X1.310. This method was stable and reliable,it could provid reference for quality evaluation of C. Oleifera.4. To explore the antihyperlipidemic effect of camellia oil on hyperlipidemia model rats. Animal models of high blood lipid of rats caused by high lipid forage were gavaged.,observation morphology changes of lipid forage and atherosclerotic plaque.The lipids of model and camellia oil groups were significantly higher than those of control group. The serum TC,TG,MDA,LDL level in the camellia oil groups were significantly reduced compared with those in model group. The aorta plaque area percentage of camellia oil group was obviously lower than that of model groups. There was no significant difference between camellia oil groups and positive controlin lowing serum lipids. Camellia oil has a marked antihyperlipidemic effect on hyperlipidmia model rats, and it could be used for preventing and treating cardiovascular and cerebrovascular disease.5. To observe the inhibiting capacity of tea saponin from Camellia Oleifera Abel in SGC-7901,MCF-7and HepG2cells in vitro.The three cells were cultured in vitro and interfered by various concentrations of tea saponin for12,24and48hours respectively.The inhibiting capacity of tea saponin in cells were detected by MTT. Tea saponin has the inhibiting capacity in cells in some degree.And A time-dependent and dose-dependent increase in cell death were observed after tea saponin treatment.The inhibting capacity increasing as the increasing doses and extension of time.The strongest inhibitory effect is in HepG2cells,IC50is20ug/mL.Tea saponin can effectively inhibit the activity of human liver cancer cells and cell proliferation, and provide experimental basis for clinical treatment of liver cancer.6. To investigate the effect of Sasanquasaponin from Camellia on the proliferation of Hepatocellular carcinoma HepG2cells and expression of Bcl-2,Bax and Caspase-3in vitro. The Hepatocellular carcinoma HepG2cells were cultured in vitro and intervened by Sasanquasaponin extract at different concentrations. After24hours, cell viability was determined by MTT assay, and mRNA and protein expression levels of Bcl-2,Bax and Caspase-3were measured by RT-PCR and Western Blot.The density of HepG2cells decreased obviously, the cells became smaller, and the proliferation of HepG2cells was inhibited by Sasanquasaponin in dose dependent manner. The expression of Bax and Caspase-3increased with the increase of the drug concentration, and the expression of Bcl-2decreased with the increase of the drug concentration.Sasanquasaponin can significantly inhibit the proliferation of HepG2cells, and induce cells apoptosis by up-regulating the expression of Bax and Caspase-3and down-regulating the expression of Bcl-2.