An Experimental Study About The Effection Of Antilogous Platelet-rich Plasma On The Revascularization Of Rabbit Prefabricated Flap

In this study, we extract platlet-rich plasma (platlet-rich plasma,PRP) from animal itself. Construct femoral arteriovenous bundle prefabricated flap on both side of these rabbits’abdomens. We randomly divided the rabbits into experimental side and blank control side. Explore the influence of prefabricated flap survive by locally using PRP. Deeply understand the role of platelet-rich plasma (PRP) from their own body in the process of prefabricated flap transfer, and further study the action principle of PRP influencing skin flap growth, providing theoretical basis for the clinical application of PRP.Construct femoral arteriovenous bundle prefabricated flap on both sides of the20healthy male New Zealand rabbits’abdomens, a total of40flaps. We randomly divided the rabbits into experimental side and blank control side, each side havings20flaps. Then we injected the autologous PRP which was prepared one day before the experiment into hypodermia around the prefabricated femoral vascular bundle in experimental side, at the same time we simply injected the same amount of physiological saline of the same amount into the control side. By using the random number table method, we divided20rabbits into4groups, each group having5rabbits. Then, we stained the prefabricated flap tissue of experimental rabbits by HE and made an immunhistochemical observation over them the7th day (group A) and the14th day (group B) after the first-stage operations. The second-stage operation hads been taken on after14days after of the first-stage operation. During the second-stage operation, we opened the island flap along the prefabricated flap marked line, that the pedicel is the implanted femoral arteriovenous bundle, then orthotopic suture the flap. Flap viability was evaluated on the7th day of the second-stage operation. And we detected the immunohistochemistry of the prefabricated flap after 7days (group C) and14days (group D) of the second-stage operation, then compared with the contralateral side, the difference of flap survival situation that Whether there is statistical significance in each time group, and discussed its survival mechanism.7days after the second-stage operation, the survival rates of the experimental sides’ flap and the blank sides’flap were (76.60±8.91)%,(64.75±6.49)%, different from both the others with p<0.05. Comparing to the control sides with microvessel density of(7.22±0.84),(9.36±0.88),(15.02±1.04), and (16.04±1.39). The microvessel density of A, B, C, and D groups in experimental sides were (10.78±1.07),(12.86±1.18),(17.42±0.84), and (18.44±0.87), compared with the control sides (7.22±0.84),(9.36±0.88),(15.02±1.04),(16.04±1.39), and all the groups were with p<0.05, and meanwhile, with the development of time, for compared the group B to the group A, and the group C to the group B, we observed the microvessel density increased significantly on experimental sides and control sides (p<0.05). The growth was not significant when compared group D to group C(p>0.05).1、Set up a simple and stable models that femoral arteriovenous bundle prefabricated flap on rabbits’abdomens;2、Autologous PRP can promote the prefabricated flap vascularization.