Study Of Bone Marrow Mesenchymal Stem Cell Transplantation In The Treatment Of Radiation-induced Lung Injury

Objective:Isolate,culture and identificate bone mesenchymal stem cells of rat, to obtain high purity BMSCs,and stability of the biological characteristics, study the BMSCs therapeutic effect on radiation-induced lung injury. Methods:1. Isolate, culture and identificate BMSCs.2. Establish radiation-induced lung injury model in rats.3. By examining serum TGF-β1, HYP levels and the SOD, MDA content in lung tissue, PCR amplification of Sry gene, to study the therapeutic effect of BMSCs on radiation-induced lung injury. Results:1. Mesenchymal stem cells was isolated and cultured by combined used the methods of density gradient centrifugation and cells adherent.BMSCs highly expressed CD29, CD90surface antigen, low expression of CD34, CD45surface antigen. BMSCs can differentiate into osteocytes and adipocytes.2.20Gy irradiation of the whole line of the chest can be successful radiation-induced lung injury for experimental models.3. In the2nd and4th weeks, A and B groups lung coefficient were statistically significant difference (P<0.05).4. Compared to group B, group A lung tissue inflammatory exudate fewer alveoli, alveolar wall structure is more complete, lesser fibrosis extent.5. Compared with group C, in the1st,2nd,4th weeks of TGF-β1levels in group A were statistically significant (P<0.05);compared with group B, TGF-β1levels of group A in the2nd week was statistically significant (P<0.05).Compared with group C, in the1st,2nd,4th weeks, HYP levels differences were statistically significant (P<0.05); compared with group B, the2nd HYP level of group A was statistically significant (P<0.05).6. Compared with group C, the SOD activity in the4th and6th weeks of group A and B were statistically significant (P<0.05);compared with group B, the SOD activity in the2nd,4th and6th weeks of group A were statistically significantly (P<0.05). Compared with group C, MDA level differences in the2nd of group A and the2nd,4th,6th of group B were statistically significant (P<0.05); compared with group B, in the4th and6th weeks, MDA levels of group A were statistically significant (P<0.05).7. In the6th week, SPB of group A expressed more than group B, the difference was statistically significant (P<0.05).8. Sry gene expression highest in the injury lung tissue. Conclutions:1. Combined used the methods of density gradient centrifugation and cells adherent can successfully isolate and culture rat BMSCs, and uniform size, power proliferation, high purity, stable biological characteristics.2. BMSCs on radiation-induced lung injury has a positive therapeutic effect.