| Objective：To explore the expression of Ufm1(ubiquitin fold modifier1) induced bymetabolic factors under diabetic condition, such as glucose、TNF-αand ox-LDL, andfurther investigate possible mechenics.Methods: RAW264.7cells were used in this stiudy. Immunofluorescence microscopywas used to observe the expression and distribution of Ufm1in Raw264.7cells. Andthen RAW264.7cells were treated with different concentrations of glucose、TNF-αorox-LDL for24h respectively. Real-Time PCR was employed to detect the expression ofUfm1, GRP78and XBP1on mRNA level, and Western blotting was applied todetermine Ufm1and GRP78expression on protein level in macrophages.Results: Ufm1was evenly expressed in both cytoplasm and nucleus in Raw264.7cells.The mRNA level of Ufm1treated with TNF-αwas significantly higher than that ofcontrol group (P <0.05). And the expression of GRP78, XBP1and Ufm1induced byox-LDL were elevated in mRNA level than that of control group (P <0.05). However,the mRNA levels of GRP78, XBP1and Ufm1incuced by ox-LDL after the treatment of4-PBA for12h were significantly lower than that were only treated with ox-LDL (P <0.05).Conclusion: Ox-LDL could induce the expression of GRP78, XBP1and Ufm1inRaw264.7cells, but inhibitor of endoplasmic reticulum stress (4-PBA) couldcompromise this effect. Therefore, we suggest that the expression of Ufm1induced byOx-LDL is at least partially due to endoplasmic reticulum stress. |
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