The Effect On The Endometrial Carcinoma Of Invasion And Metastasis Ability By Interferenceing The Expression Of The Snail Gene

Part I Expression of Snail protein in endometrial carcinoma and its clinicalsignificanceObjective: The relation between the expression of Snail in endometrial carcinoma toexplore Snail and endometrial cancer invasion and metastasis and prognosis.Method: Immunohistochemistry and tissue microarray were employed to evaluateSnailxpression in124endometrial carcinoma,28atypical hyperplasia and35normalendometrium samples. Then the clinicopathological significance in the invasion andmetastasis of endometrioid carcinoma was analyzed.Results: The presence of Snail protein was46.8%in endometrial carcinoma,respectively. Compared to atypical hyperplasia and normal endometrium, there was astatistical significance (P <0.01). And increased expression of Snail was associatedwith FIGO stage and lymph node metastasis. Patients with positive expression ofSnail had worse survival than those with negative one.Conclusion: Snail could regulate the expression of HIF-1α and E-cadherin, play animportant role in endometrial carcinoma and in the process of invasion and metastasis,the intervention of Snail pathway may provide a new method for the scheme ofdiagnosis and treatment of uterine endometrial carcinoma. Part II ShRNA inhibited the expression of Snail geneObjective: Inhibition of expression of the transcription factor Snail through shRNA,observation of human endometrial carcinoma ISK cells in vitro invasion and epithelialmesenchymal transition relation.Methods: Construction the expression of Snail short hairpin RNA (Smallhairpin-shaped RNA, shRNA) RNA interference vector (Snail shRNA vector) and theexpression does not match any known mRNA shRNA negative control vector of RNAinterference (control shRNA vector), respectively, in ISK cells transfected with Snailexpressing ISK-shSnail cells and screened by inhibiting the expression of Snail wasnot affected. ISK-shControl cells. Using RT-PCR and Western blot at mRNA andprotein levels were verified. With the proliferation of CCK-8cells were detected.Method of Matrigel combined with Transwell cells compared the difference betweenthe groups of cells in the migration and invasion.Results: Snail-shRNA group and non transfection group compared to ISK, Snailexpression was significantly decreased (P <0.01), Transwell chamber transmembranecell number was significantly reduced (P <0.01); group ISK-shControl, Snailexpression and Transwell chamber wore number and ISK membrane cellsuntransfected group than significant difference (P>0.05). The cell growth wasmeasured by CCK-8assay, ISK cell growth slowed down after Snail interference, andnon transfected group was significant.Conclusion: Expression by RNA interference silencing Snail gene can effectivelyinhibit ISK in endometrial cancer invasion and migration ability, at the same timereversal of epithelial mesenchymal transition, the intervention of Snail pathway mayplay an important role in diagnosis and treatment for endometrial cancer.