Studies On The Anti-Tumor Effect Of Surfactin On HepG2Cells

In this study, the effect of surfactin on Human hepatoma cell line HepG2proliferation and apoptosis-related mechanism were studied.Cell viability was detected by MTT assay. The results showed that surfactin could inhibit the proliferation of HepG2, the IC50was42μg/ml after being treated24h. And L-02cells were observed at low apoptosis rate. MTT assay results showed that surfactin could induce apoptosis in HeG2cells specifically.HE and Hoechest33342staining were used to observe the cell morphology. The staining results showed typical morphological changes, such as cytoplasmic concentration, nuclear disintegration. The result of FCM testing illustrated that cell apoptosis rate was up to15.7±1.21%after treated with surfactin for48h, and the cells were arrested at G2/M phase. The cell morphology testing showed that surfactin could induce apoptosis in HepG2cells through arresting at G2/M phase.Laser scanning confocal microscope (LSCM) testing illustrated that:ROS was significantly increased within4h. The generation of ROS could down-regulate the [Ca2+]c level. The [Ca2+]c generation was depended on the IP3R and RyR opening; ROS and [Ca2+]c could attack the mitochondria and endoplasmic reticulum, then induced the PT pore opening, resulting in the reducement of ΔΨm and ERS.The western blotting and RT-PCR revealed:the expression of P-ERK was down-regulated after treatment with surfactin, which was regulated by [Ca2+]c. The expression of Bax/Bcl-2was regulated by p-ERK.