Isolation And Identification Microorganisms Transformation The Organic Iron And Zinc And Study On The Activity Of Pharmacology

Iron and zinc are essential nutrient for the human and animal, and they are closely related to many basic physiological activities. The deficiency of iron may result in anaemia, and zinc deficiency may result in physical, mental retardation and low immunity.Many microorganisms can accumulate microelements including iron and zinc, and transform inorganic compound to organic compound, which is beneficial to people and animals absorption efficient. It is an effective measure to develop new supplement of iron and zinc, which are obtained by biotransformation.By microbial transformation, especially the use of saccharomyces cerevisiae transformation can obtain organic iron and organic zinc, which are low toxicity and absorption effective. However, the problems that low transformation efficiency and genetic instability are already existence. This study isolates high-iron-enriched strain and high-zinc-enriched strain from natural environmentThe study obtains the iron-enriched strain BY-1109and zinc-enriched strain LZ-1108. The iron-enriched strain BY-1109tolerates iron with10000mg/L, and the iron content in the cell is10.23mg/g. The high-enriched strain LZ-1108tolerates zinc with8000mg/L, and the zinc content in the cell is6.7mg/g.This study identifies the iron-enriched strain and zinc-enriched strain by the morphology and molecular biology, and optimizes the fermentation condition. The result of the partial18S rDNA region of iron-enriched strain alignment based on BLAST analysis reveals that the strain is identified as Rhodotorula. And the morphology of high-iron-enriched strain is consistent with Rhodotorula. The optimal fermentation condition of the iron-enriched strain is initial pH7.0with Fe2+8000mg/L at30℃for72h. The result of zinc-enriched strain is identified as Fusarium. And the morphology of zinc-enriched strain is consistent with Fusarium. The optimal fermentation condition of the strain is initial pH7.0with Zn6000mg/mL at28C for72h. However, Rhodotorula could not grow on the medium with inositol, the study find that iron-enriched strain is clearly different and could be incubated with inositol. There is need further researches to make sure whether the strain would be possibly anamorphic yeasts of the genera Rhodotorula or a novel species.The study analyses iron-enriched strain transform the inorganic iron to organic iron and the distribution of iron in cells. The cells growing in the medium containing Fe2+, iron content in cell walls, vacuolar, cell membranes and mitochondrial fractions are significantly higher than that in the cells grow in the medium without Fe2+particularly in cell walls, cell membranes and vacuoles. This indicated that inorganic iron in medium are transported into the cells and are converted into organic iron compounds.In the study, the iron-enriched strain and zinc-enriched strain are used to take bioavailability tests. The whole blood is investigated by flame atomic absorption method after gavage administration to rats at a single does of10mg/kg. The peak plasma concentration of iron-enriched strain and zinc-enriched strain are attained at48.07min and14.04min, respectively, and Cmax are901.27±63.73μg/mL and23.72±4.47μg/mL, respectively. The bioavailability of the two strains is120%and189%.