The Experimental Study Of HUC-MSCs Complex Chitosan Conduit To The Inducing Different Growth Of The Nerve Lateral Bud

ObjectiveThe issues contemplated by bridging the human umbilical cord mesenchy-mal stem cells (HUC-MSCs) chitosan composite nerve conduit, establish-ment of a new tibial nerve-peroneal nerve anastomosed in animal models,a series of detection research, access to data and analysis, in order to verify chitosancomposite conduit combined with HUC-MSCs in the role of Neurorrha-phy, confirming and promoting the role of the composite nerve conduit in nerveregeneration, and to find a new way to promote nerve lateral bud germination andeffective differentiation to provide experimental evidence for the longer defects ofperipheral nerve in clinical treatment。MethodsApplication of the chitosan biological characteristics in making nerve conduit, theconduit is filled with human umbilical cord mesenchymal stem cells (HUC-MSCs), bridging nerve end-to-side anastomosis. The experimental animals wererandomly divided into three groups,10in each group: A group (control group): thetraditional tibial nerve-peroneal nerve end-to-side anastomosis group; group B(experimental group): tibial nerve-peroneal nerve bridging chitosan nerve conduitend-to-side anastomosis group. Group C (test group2): tibial nerve-peroneal nervebridge filled chitosan HUC-MSCs composite nerve conduit side anastomosis group. Usinggeneral observation, neurophysiological and histological observation and anti-S-100immunohis-tochemical detection indicators, the detection data were statistically analyzed. ResultsAll experimental animals survived the surgery， no incision site infection, nervecontinuity was good， minor adhesions with the surrounding tissue and easily separated。12weeks after surgery chitosan composite nerve conduit completely degraded。Electrophysiological testing showed that the three groups of transplanted nerves restoredthe ability of electrical conduction, and nerve conduction velocity。 Differences betweenGroup C and A，Group C and B were statistically significant (P <0.01), the group A andgroup B difference were not statistically significant, group C nerve recovery was best, andworst among group A. Toluidine blue staining and transmission electron microscopyshows that the three groups A, B, C in the number of myelinated nerve fibers and myelinthickness were statistically significant (P <0.01), group C optimum,and group A worst.Anti-S-100Immunohistoche-mistrymethod brought significant difference between thethree groups A, B, C in Schwann cell number, shape and arrangement。 The growth ofcells， shape and arrangement in group A was worst and optimal in group C.ConclusionsHuman umbilical cord mesenchymal stem cells (HUC-MSCs) chitosan compositenerve conduit has a significant role in promoting nerve regeneration in nerve end-to-sideanastomoses， inducing shaft bud growth, accelerating the growth rate of regeneratingfibers, promoting the growth and maturation of Schwann cells.