The Shou Wu Yan Shou Dan Protection Mechanisms Study Of The Rat Brain Tissue During Cerebral Ischemia Reperfusion Injury

ObjectiveTo study the effects of Shou Wu Yan Shou Dan (SWYSD) on the proteinexpression of tumor necrosis factor-α (TNF-α), leukocyte interleukin-1β (IL-1β)and intercellular adhesion molecule-1(ICAM-1), and to explore the protectiveeffects mechanism of SWYSD on ischemia-reperfusion injury in rat brain tissue.Methods80healthy adult SD rats, male, were randomly divided into sham operationgroup, cerebral ischemia reperfusion group, the low and high dose groups ofSWYSD and nimodipine group,16rats in each group. Then saline were given tothe sham operation group and cerebral ischemia reperfusion group at10ml/kgweight; the nimodipine group were given to nimodipine at12mg/kg weight; thelow and high dose groups of SWYSD were given containing crude drug of15.3g/kg, decoction of76.5g/kg. All of these medicines were given to rats at10ml/kg weight and one time each day, for7consecutive days, the normal diet.Preoperative fasted for12h, used the internal carotid artery line occlusion to theestablishment of the middle cerebral artery occlusion cerebral ischemia reperfu-sion model. Except the sham group, the remaining4groups embolization of the left middle cerebral artery, after90min of ischemia and reperfusion for24h.After reperfusion, carry out Neurological deficits symptom score; observed HEstaining brain pathological changes under light microscope; immunohistochemi-stry and Western Blotting detect the protein expression of TNF-α, IL-1β andICAM-1in brain tissue.Results1. Neurological deficits symptom score: Sham-operated rats no significantneurological deficit symptoms; Cerebral ischemia-reperfusion group than in thesham group showed a significant neurological deficit symptoms, there is a stati-stically significant (P<0.01); Compared with the Cerebral ischemia reperfusiongroup, the high and low groups of SWYSD and nimodipine group of neurologicaldeficit symptoms were significantly improved, and the difference was statisticall-y significant (P<0.01).2. Pathomorphology changes: Sham-operated rats normal brain structure,uniform density, normal cell morphology, no significant histological changes;Cerebral ischemia reperfusion group ischemic side cortex loose organizationalstructure, disorganized, severe edema of the cell, the state is unclear, cellvacuolar degeneration, cytoplasm color deepened, a large number of nervecells showed triangle, a part of karyopyknosis, cell necrosis; The high and lowgroups of SWYSD and nimodipine group ischemic cortex brain than regularstructure of the organizational structure, the cells arranged in order, a clearerboundary, compared with the cerebral ischemia reperfusion group, neuronaldegeneration, necrosis, the vacuolar degeneration significant improvement.3.Immunohistochemical method and Western Blotting method show: Compar-ed with the sham group, cerebral ischemia reperfusion group the proteinexpression of TNF-α, IL-1β and ICAM-1in brain tissue were increased, thedifference was statistically significant (P<0.01); Compared with the cerebralischemia reperfusion group, the low and high dose group SWYSD and nimodipine group the protein expression of TNF-α, IL-1β and ICAM-1have adifferent degree of reduction, and the differences were statistically significant(P<0.01).ConclusionsSWYSD on cerebral ischemia reperfusion injury in rats by down-regulatingthe protein expression of TNF-α, IL-1β and ICAM-1in brain tissue to inhibit theinflammatory reaction occurred. Its mechanism may be associated with inhibitthe secretion of inflammatory cytokines and expression, and to reduce theinflammatory response of brain tissue.