PIWIL4Gene Regulates Growth Of Cerviacal Cancer HeLa Cells

Aims PIWI, a significant plasmosin, is involved in many physiological and pathological progresses, including spermatogenesis, RNA interfere and translation regulation. It has been certified that the human PIWI family is related with a variety of tumors.PIWI may function as an oncogene in the process of tumorigenesis.We also found that PIWIL4is overexpressed in ovarian cancer and inhibit ES-2cell growth and proliferation activity by RNAi. However, the function of PIWIL4in cervical cancer has not been reported. Our aim is to detect PIWIL4gene expression in cervical cancer,and to further research the effect of PIWIL4to HeLa cell growth phenotype, to explore the function of PIWIL4and its regulation mechanism.Methods Through the real-time PCR tech, to test the level of PIWIL4is expressed in cervical cancer tissue. Using the MTT, colony formation, and growth curve experiments to detect the variation of growth activity in human cervical squamous tumor HeLa cell after overexpression or knockdown of endogenous PIWIL4. Then, the predicted mechanism was verificated by TUNEL to detect the cell apoptosis and western blot to detect the protein level of caspase-8and caspase-9.Results PIWIL4is upregulated in the cervical cancer tissues,about77.8%. In the HeLa cells, the growth and colony formation abilities were upregulated after the overexpression of the PIWIL4gene, while the results were the opposite as the knockdown of PIWIL4by si-PIWIL4. Through the TUNEL tech, we found that PIWIL4could inhibit apoptosis and the protein expression levels of caspase-8and caspase-9, maybe as a oncogene.Conclusions PIWIL4is upregulated in the carcinoma of cervical, In HeLa cell, PIWIL4can promote the cell growth activity, may as a oncogene through the inhibition of apoptosis. According to the study of the molecular mechanism of PIWIL4, we learn more about the tumorigenesis, and it provides new clues for PIWIL4to be an important target of early diagnosis and treatment.