Study Of Apoptosis In The Trerapeutic Of HaoBieYangYin RuanJian Prescription On Schistosomiasis Liver Fibrosis In Mice

Objective:To explore the therapeutic of HaoBieYangYinRuanJian Prescription on hepatic fibrosis of schistosomiasis in mice and determine the apoptosis of hepatic stellate cells.Methods: Every mouse were infected by25schistosoma cercarias through skin of abdominal wall, hepatic fibrosis was induced after12weeks. The infected mice were randomly divided into8groups: HaoBieYangYinRuanJian Prescription low, middle and high dosage group, positive control groups (FuFangBieJiaRuanGan tablet control group and Colchicine control group), model control group(3months group after infection,6months group after infection) and abnormal control group. HaoBieYangYinRuanJian Prescription groups and positive control groups were given powder extractive suspension orally once a day. Model control group was given normal saline orally once a day. Intragastric administration lasts12weeks. Hepatic tissue was obtained at the end of the experiment to observe the pathological change under microscope, determine the content of Hyp and set out a-SMA expression and cell apoptosis using immunohistochemical method.Results:(1)Collagen staining biopsy: Hepatic pathological changes of model control groups (long model, short model) are severe, forming typical hepatic fibrosis of schistosomiasis. HaoBieYangYinRuanJian Prescription can significantly alleviate pathological changes. The stage and score of hepatic fibrosis in model control groups (long model, short model) is significantly higher than that of the normal group respectively (P<0.01). Each treatment group were significantly lower than that of long model (P<0.01). high dosage group of HaoBieYangYinRuanJian Prescription is lower than that of FuFangBieJiaRuanGan Tablet control group and Colchicine group (P<0.01). While its fibrosis score is lower than that of Colchicine group(P<0.05).(2) Collagen content of hepatic tissue: Compared with the normal control group, collagen content of model control group (long model, short model) hepatic was significantly increased (P<0.01). Compared With the model group (long model), collagen content of high and middle dosage group of HaoBieYangYinRuanJian Prescription is significantly lower (P<0.05).(3) Effect to a-SMA expression for hepatic stellate cells(HSC) of hepatic fibrosis of schistosomiasis: Normal control group has no marked difference with treatment group under microscope by immunohistochemical staining. a-SMA of HSC expressed a lot in model groups, which demonstrated Schistosomiasis induces the activation of HSC. a-SMA expression of HSC in treatment groups was evidengly reduced, reminding HSC activation was inhibited.(4) Inspection of HSC apoptosis:The cells showing brown granules in nucleus are apoptotic cells. The quantity of HSC in middle dosage group of HaoBieYangYinRuanJian Prescription is significantly different to that of model group (P<0.05), but has no remarkable difference with normal control group(P>0.05).Conclusion: The model of hepatic fibrosis of schistosomiasis in mice was successful. HaoBieYangYinRuanJian Prescription has therapeutical effect to hepatic fibrosis of schistosomiasis in mice. It can significantly alleviate the degree and range of hepatic fibrosis, reduce collagen content and a-SMA level of hepatic tissue and induce HSC apoptosis.