Expression Of Flot-2in Human Gastric Cancer And Its Effect On Cell Proliferation And Invasion

Objective:To study the expression of Flot-2in human gastric cancer and its effect on cell proliferation and invasion.Methods:1.The expression of Flot-2in90specimens of gastric cancer tissues and90spceimens of adjacent tissues was detected by immumohistochemistry.The relationship between their expression and clinicopathological fectures,prognosis was statistically analyzed.2.The specific Flot-2small interference RNA(siRNA) was transfected into gastric cancer cells using lipofectamineTM2000.Three groups of cells were used in the studies,including Flot-2siRNA group(AGS and SGC7901cells transfected with the Flot-2siRNA), negative control group(AGS and SGC7901cells transfected with the non-silencing siRNA) and blank conrol group (untransfected AGS and SGC7901cells).The expression of Flot-2mRNA was detected by RT-PCR.Cell proliferation was assessed using MTT assay.Transwell analysis was done to measure the invasion of cells.Results:1. The expression of Flot-2in gastric cancer tissues was significantly higher than that in adjacent tissues (P<0.05).Overexpression of Flot-2was statistically significantly associated with pathological grade,depth of invasion,lymph node metastasis status,TNM stage(P<0.05).Moreover, a significant positive correlation was observed between Flot-2expression and pathological grade(r=0.370, P<0.001), depth of invasion(r=0.391, P<0.001),lymph node metastasis status(r=0.526, P<0.001),TNM stage (r=0.720, P<0.001).In univariate analysis, Overexpression of Flot-2was associated with poor5-year survival rate(P=0.000).In Cox multivariate analysis,expression of Flot-2was found to be one of the independent prognostic factors(P<0.05).2.Compared to negative control group,the mRNA expression of Flot-2in Flot-2siRNA group was decreased significantly(P<0.01).The proliferation and invasion of cells were significantly decreased in Flot-2siRNA group(P<0.01),compared to negative control group and blank control group.Conclusions:(1)Overexpression of Flot-2is closely related with invasion and metastasis of gastric cancer,and may be regarded as factors of poor prognosis in gastric cancer.(2)Silencing of Flot-2expression can inhibit the proliferation,invasion in AGS and SGC7901cells.