Study On Correlation Of Lesions Development Between Live Tissue And Intestinal Mucosa Schistosoma Japonicum Infection Mouse Model

Object:Establishing different disease stages of mice model infected with Schistosoma japonicum, this study aim to explore lesions development correlation between live tissue and intestinal mucosa, and to provide new practical technology and theoretical foundation for judging the severity and prognosis of Schistosoma japonicum. Methods:50healthy male Kunming mice were purchased from a professional laboratory animals company.10Kunming mice were randomly selected as control group, rest40Kunming mice were infected with Schistosoma japonicum cercariae by skin, with20tails per mouse. After infection, the mice were fed by conventional sub-cage. After12weeks,10Kunming mice were randomly selected for a drenching insecticidal treatment with praziquantel (300mg/kg.d). Excepting the10mice, in the infection cycle of6weeks,12weeks and21weeks,10Kunming mice were randomly selected for observing, as well as the mice which were treated9weeks. Serum was collected in designed time point for TGF-betal and ATPase detecting by ELISA method. Dissect mice, carefully check schistosome parasites in the hepatic portal vein and superior mesenteric vein and record the number of parasites per mouse. After observing and recording the whole live and colon specimens of mice, the specimens were embedded in paraffin and executed a routine HE staining, then, the histopathological changes between which were observed and analyzed.Results:(1) In different disease stages of mice model infected with Schi-stosoma japonicum, the success rate is100%; At6weeks, mouse model began to appear granuloma. At12weeks, mouse model began to appear liver fibrosis. At21weeks, mouse model began to appear liver cirrhosis. Spearman rank correlation analysis showed that the concordance between colon histopathological scores and liver pathological grades was good (r,=0.892, P<0.01).(2) The ATPase concentration in control group, group6w, group12w, group21w and treatment group are207.74±4.98nmol/L, 302.96±5.54nmol/L,501.72±7.12nmol/L,505.01±5.85nmol/L,211.85±5.51nmol/L respectively. Excepting control group between treatment group, group12w between group21w, statistically difference was significant in the rest of the groups (P<0.05).(3) The TGF-betal concentration in mice serum is rising with the infection period. The TGF-betal concentration decrease significantly after treatment. There is no statistically significant difference between treatment group and control group(P>0.05). Conclusions:In different disease stages of mice model infected with Schistosoma japonicum, colon histology score of mouse is corresponding with liver pathology classification group. TGF-betal and ATPase in serum is increased with the extension of the infection period, reduced after treatment, suggesting which is potential in assessment for disease assessment.