The Effects Of Cell Proliferation And Apoptosis On Colorectal Rcinomaca Treated By COX-2Selective Inhibitor Combined With Oxaliplatin

Colon cancer is a common digestive tract cancer, The traditional principles of treatment are comprehensive treatment based on surgery, combined with chemotherapy and radiotherapy to reduce the recurrence rate and improve survival. Comprehensive treatment in chemotherapy, occupies an important position for recurrent or metastatic colon cancer. Recent studies have found that cyclooxygenase-2(COX-2) may be involved in tumorigenesis through a variety of channels, such as accelerating progression, inhibiting apoptosis and or promoting angiogenesis. The experiment studies the influence on cell proliferation and apoptosis of colon cancer HCT-8cells cultured in vitro study, by oxaliplatin alone, COX-2inhibitor NS-398and oxaliplatin combined with NS-398.Preliminary view of the combined effects of capecitabine platinum and NS-398to HCT-8cells was observed under an inverted microscope to see the influence on growth of HCT-8cells, of oxaliplatin and oxaliplatin combined with NS-398and found that oxaliplatin the platinum and the NS-398concentration increased gradually the reduction HCT-8cells, and make them round, adherent cells floating refraction.Then, the functions of different concentrations of CCK-8assay oxaliplatin alone (1.25,2.50,5.00,10.00,20.00mg·L-1), oxaliplatin combined with NS-398(20.00,80.00μmol·L-1) to24h HCT cells inhibition rate are tested; Annexin V-FITC and PI double staining flow cytometry is used to test the conditiond of early apoptosis of HCT-8cells in48h when treated with oxaliplatin (10.00mg·L-1).NS-398lespectively and together; and analyzed by flow cytometry HCT-8cells’24h cell cycle changes.were analyzed oxaliplatin, NS-398alone and Oxaliplatin in combination with NS-398. after becrg treated with (2.50,5.00,10.00,20.00mg·L-1) For further researching of capecitabine platinum and NS-398the synergistic effect to the cells, use agarose gel electrophoresis oxaliplatin alone and combined with NS-398(10.00,100.00μmol·L-1) by fluorescence microscopy cell apoptosis of HCT-8cells at48h the DNA Ladder situation and48h apoptosis. Use caspase-3activity of oxaliplatin and NS-398alone and combined to see the effects on colon cancer HCT-8cells48h change of Caspase-3activity.The experiment found that oxaliplatin and NS-398showed a dose-dependent inhibition of HCT-8cells (P<0.05). Cell proliferation of Oxaliplatin combined with NS-398treated group inhibition was significantly higher than the only concentration of oxaliplatin, and it increased with the added dose of NS-398. For the Annexin V-FITC detection of early apoptosis, oxaliplatin dose-dependent induction of apoptosis and cell death, a joint action group of HCT-8cells was significantly stronger than with oxaliplatin role of HCT-8cells, the difference was statistically significant (P<0.05); cell cycle showed that oxaliplatin, NS-398and combined effects of group HCT-8cells after48h are different. So the cell cycle can be blockde, oxaliplatin canblock HCT-8cells in S phase arrest, NS-398HCT-8cells G0/G1arrest, oxaliplatin and NS-398can induce apoptosis in colon cancer HCT-8cells, add the NS-398The oxaliplatin apoptosis significantly increased. Agarose gel electrophoresis showed able to detect DNA Ladder. in colon cancer HCT-8cells48h it was the combined effects of oxaliplatin, NS-398with the help of NS-398added oxaliplatin of different doses and DNA ladder was brighter than oxaliplatin Platinum group. When observing apoptosis at48h with Hoechst33258fluorescence microscopy, normal nuclei were uniform and dispersed blue, the nuclei of apoptotic cells were dense stain, or was chunky, some white color could be seen After apoptotic cells were significant after covnbined therapy. Caspase-3activity of testing oxaliplatin and NS-398used alone increased in colon cancer HCT-8cells and it could be. Strengthened from a combination of two drugs, in colon cancer HCT-8cells. The difference was statistically significant (P<0.01), the two had a synergistic effect.COX-2inhibitor NS-398, oxaliplatin inhibited the growth of colon cancer cells in a concentration-dependent manner. The same concentration of the combined effects of the two drugs to HCT-8cells can inhibit the growth of and induction of apoptosis. The combined effects of the two drugs have obvious synergies. Oxaliplatin in combination with the COX-2inhibitor NS-398anti-tumor mechanism are related with cell cycle and Caspase-3-induced apoptosis pathway. So it can be seen that COX-2selective inhibitors in combination with L-OHP and other chemotherapy drugs have a wide range of applications, providing hope for the clinical dose of drugs.