Study Of Renal Cortical Perfusion In Rabbit By Microbubbles-enhanced Ultrasound Cavitation

Objective: To explore the change of renal cortical perfusion in Rabbit throughMicrobubbles-enhanced Ultrasound Cavitation.Methods:20New Zealand rabbits were randomly divided into four groups(N、B、C、D,5rabbits in each group), the N group as the control group, SonoVue was injectedvia the ear vein at a dose of0.1ml/kg. The rabbit kidneys were irradiated with differentmechanical indexs(MI) for60s under the mode of contrast enhancement, after15minute, conventional ultrasound contrast was made again and then draft and analysistime-intensity curves(TIC）, parameters including: time to peak(TTP), the slope rate ofdescending curve(α), the slope rate of ascending curve(A), derived peak intensity(DPI),and area under the curve(AUC). Blood samples were drawn from the ear vein to testserum creatinine(CRE), blood urea nitrogen(BUN). After the experiment the rabbitswere killed, to obtain kidney specimens, HE staining and light microscopy to observethe morphological change.Results: Kidney perfusion curve toward similar to the gamma curve, resultingrenal perfusion results of quantitative parameters. Compared with the N group, the A, α,DPI of the C and the D group were significantly decreased(P<0.05), while the AUCwas significantly increased(P<0.05) and the TTP was significantly prolonged(P<0.05).There were statistically significant differences, and no significant changes in B group(P>0.05). Compared with the B group, the DPI, αof the D and the C group weresignificantly decreased(P<0.05), while the AUC was significantly increased(P<0.05)and the TTP was significantly prolonged(P<0.05)Conclusions: Under the microbubbles-enhanced ultrasound cavitation, the changeof hemodynamic of the rabbit renal cortical perfusion can be observed. To providesecurity guidance and experimental evidence for the safety of SonoVue and the furtherclinical application of diagnostic ultrasound cavitation effect on the extent of tissue damage.