Vitiligo Clinical Research And Clinical Characteristics Of The Formation Of Myosin X Influence Cell Filopodia Formation In Human Keratinocytes

Objective:Clinical characteristics of occurrence of vitiligo, and to explore the effects ofmyosin x cell filopodia formation in human keratinocytes.Methods:1.Clinical study of vitiligo pathogenesis through clinical observation formcharacteristics of vitiligo.2.With the aid of small interfering RNA (small interfering RNA, siRNA)-mediated RNA interference (RNA interference, RNAi) technology, using Myo x siRNAgene transfection of HaCat cells, and RT-PCR and Western blotting method to observethe changes of HaCat cells in Myo X gene mRNA and protein levels of transfectionbefore and after treatment.3.To observe the morphological changes of HaCat cell filopodia and Myo x siRNAgene transfection with scanning electron microscope.Results:1.Clinical observation on200cases of patients with vitiligo, lack of86%patientswith trace elements, in which copper and selenium deficiency is heavy.2.The designed siRNA can effectively down-regulate Myo X gene mRNA andprotein expression level.3.Scanning electron microscopic observation of siRNA transfectiondown-regulates the expression of Myo gene in HaCat cells and the number of filopodiareduced.Conclusion:1. Related to the lack of trace elements and the incidence of vitiligo.2.The formation process of Myo x involved in HaCat cell filopodia, inhibit the expression of Myo X in HaCat cells, HaCat can reduce the formation of filopodia.