Effect And Mechanism Of Colchicine On Human Breast Cancer MCF-7Cells And MDA-MB-231Cells

The purpose of this project is to through theoretical studies and in vitro experiments to explore colchicine inhibit the proliferation of breast cancer cells, promote the role and mechanism of apoptosis, on the basis of the foregoing experiment, to further explore the TCM etiology and pathogenesis of breast cancer, and to clarify the TCM etiology the molecular mechanism of the disease the rule is reasonable and anticancer detoxification, anti-breast cancer clinical applications provide new possibilities.Theoretical studies, first an overview of the progress of modern medical treatment of breast cancer. The second section describes the Chinese understanding of the etiology and pathogenesis of breast cancer, from which to extract the "cancer drug" special pathological factors of cancer drug and breast cancer development, treatment, prognosis, and important rule of established breast cancer One of the detoxification cancer. Finally, the research progress of the the mentor an overview of commonly used the detoxifying herbs optical arrowhead in the treatment of breast cancer clinical practice and extract colchicine prime anticancer mechanism.Experimental studies, the mechanism for colchicine treatment of breast cancer in vitro experiments. Experiment:MTT method was used to observe the colchicine inhibition of human breast cancer MCF-7cells and MDA-MB-231cell proliferation. Results:With the increase of the concentration of colchicine and medication time extend the value-added of the inhibition of breast cancer MCF-7cells and MDA-MB-231cells gradually increased. Compared with the negative control group, in addition to a dose of0.3125ug/ml24hours0.625ug/ml, the duration of action of the two groups no significant remaining groups have a significant statistical significance (P<0.01). Compared to the other two groups of cells, the same concentration of colchicine inhibition of MDA-MB-231cells was higher than the same time after breast cancer MCF-7cells. Experiment:flow cytometry of colchicine on human breast cancer MCF-7cells and MDA-MB-231cell apoptosis. Results:human breast cancer cells MCF-7and MDA-MB-231cells after different concentrations of colchicine factors for24h,48h, after72h of each group showed a different degree of apoptosis and apoptosis rate increasing dose extension of time gradually increased. Compared to the two types of cells, the same concentration of colchicine after the same time breast cancer MDA-MB-231cells in the total rate of apoptosis in breast cancer MCF-7. Experiment:flow cytometry colchicine on human breast cancer MCF-7cells and MDA-MB-231cell proliferation cycle. Results:colchicine40ug/ml amount for72h with a significant difference, both apoptosis highest rate,18.97%,9.81%, compared with the normal control group, G0/G1phase cell ratio with the prolonged duration G2/M phase of the cell ratio with the role of time gradually reduce gradually increase, obvious cell cycle arrest at the G2/M phase, with obvious time-dependent manner. Conclusion:colchicine can inhibit human breast cancer MCF-7cells and MDA-MB-231cell proliferation, induction of apoptosis, with a significant dose-and when-effect relationship. Cell proliferation inhibition and induction of apoptosis may be associated with the G2/M phase. The two types of cells and results compared to colchicine inhibition of value-added role and ability to induce apoptosis of human breast cancer MDA-MB-231cells.