Preparation And Characterization Of Composite Scaffolds By Laser Melt Electrospinning

Scaffold plays an important role in tissue engineering strategies, which is directly related toimportant progress and breakthrough in the ifeld of tissue engineering. Laser meltelectrospinning as a novel environment-friendly and high efifciency method to preparemicro/nano reticular formation compared to the other electrospinning, which can prepare higherarea and porosity of the three-dimensional network structure by adjusting the spinningparameters. The scaffolds have the ability to mimic the biological function and structure ofnature extracellular matrix (ECM). Meanwhile, laser melt electrospinning has become a hot topicof research due to combination the advantages of the inorganic/organic composite nanoifbers.In the present paper, the polylactide (PLLA)，poly (s-caprolactone)(PCL)，andnano-hydroxyapatite (nHA) were chosen as tissue engineering scaffold materials. The physicalproperties, biodegradability and compatibility of biological were studied and the applicationprospects of the ifber scaffold were made preliminary study1.The PLLA/PCL and PLLA/PCL/nHA composite ifbers scaffold were prepared by the lasermelt electrospinning method. The porous structure of the ifber scaffold, the addition of the nHA,the structure and the thermal properties were analyzed by using SEM、EDS、XRD and DSC. Thevitro degradation and biocompatibility evaluation experiment on the ifber scaffold were alsocarried out. The experiments showed that the cell survival rates of the HEK293on the PLLA andPLLA/nHA(7%) ifber scaffold were96.84%and106.1%repectively. The survival rate of thecells cultured on the membrane scaffold PLLA/nHA composite ifbers was increased comparedwith the blank test.2.The PCL and PCL/nHA micro/nano-composite ifber scaffold were also prepared by thelaser melt electrospinning method. The micro morphology，elemental content, crystallinity,thermal stability, crystallinity and mechanical properties of ifber scaffolds were analyzed byapplying the SEM、EDS、XRD、DSC and mechanical testing. The results exhibited that the elastic modulus of the composites was maximum at63.56Mpa when the nHA content reached7%，the breaking elongation rate was343.23%when the nHA content come to9%，the maximumtensile strength of3.32MPa was achieved when the nHA content reached3%，the XRD analysisindicating that the best dispersion was gained when the nHA content was7%. The cell survivalrates on the PCL and PCL/nHA (7%) fiber scaffold were93.56%and102.7%，separately.3. In order to make full use of the advantages of the PLLA、PCL、nHA, the “sandwich”structure of PLLA/PCL and PLLA/PCL/nHA were constructed. The morphology and hydrophilicproperties of the ifber scaffold were analyzed by the SEM and WCA, the cell survivals were113.2%and97.75%，separately.