| Objective To observe the influence of all-trans retinoic acid(ATRA) on the process of neointimal formation and the expression of CyclinD3and Lipoprotein-associated phos pholipase A2in Rabbits’Carotid Atherosclerosis.[Methods] Fifty-six healthy male New-Zealand rabbits were randomly divided in to9groups(=6):normal control groups(A, B, C), high-fat groups(A, B, C), treatment groups(A, B, C). There were6rabbits in each group. The normal control groups were fed with conventional chow, and underwent surgery without endothelium injury. The high-fat groups were fed with high cholesterol chowand.subjected to the carotid endothelial injury by air desiccation. The treatment groups were a dministered ATRA daily by gavage, and other measures were the same as the high-fat groups. The rabbits in the A, B and C groups were sacrificed respectively at7,14and28days after surgery. The carotid artery segments were harvested for histomorphometry observation and determination, and immunohistochemistry and ELISA were used respectively to detect the CyclinD3and Lipoprotein-associated phos pholipase A2.[Results]①In the normal control groups, neointimal formation was not found and the expression of LP-PLA2was microscale. CyclinD3could not be detected in the target vessels.②In the high-fat groups, there was a distinct intima hyperplasia and narrowed luminal area at twenty-eight days after surgery.The expressions of CyclinD3and LP-PLA2were significantly intense.③In the treatment groups, the CyclinD3and LP-PLA2expression was markedly lower than that in high-fat group(P<0.05). And there was an outstanding smaller intimal/medial area ratio and larger luminal area in treatment groups(p<0.05).[Conclusions] The results showed that ATRA could inhibit LP-PLA2and CyclinD3expression,thus it could reduce inflammation and VSMC proliferation and inhibit intima proliferation and elicit favorable geometric remodeling of the carotid atherosclerosis in rabbits,... |
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