Study On The Correlation Between MiR-145and PI3K/AKT Signaling Pathway In Epithelial Ovarian Cancer Tissue

Objective：1.To examine the expression of miR-145,Akt and p-Akt protein in Normal ovary,benign and malignant epithelial ovarian tumor tissues.2.To explore the roles of miR-145,Akt and p-Akt protein in the tumorigenesisand progression of human epithelial ovarian cancer, and the relationship amongmiR-145,Akt and p-Akt protein in the carcinogenesis of ovarian cancer。Methods：A total of10normal ovarian cases,19benign epithelial ovarian tumor cases and17epithelial ovarian cancer cases were collected from Maternal and Child HealthHospital of Jiangxi Province.The expression of miR-145was examined by semiquantitative RT-PCR,and Western blot was used to measure Akt and p-Aktexpression.meanwhile, we analyzed these data with statistical software.Results：1.The expression of miR-145was detected in normal ovarian tissue,benign andmalignant epithelial ovarian cancer tissues.and the expression level presented withsignificant differences (F=12.267,P<0.05).MiR-145expression in epithelial ovariancancer group was lower than that in other histologic groups (P<0.05).The expressionlevel of miR-145has no relation with FIGO stage and pathologic types(P>0.05).2.The Akt protein was expressed in normal ovarian tissue, benign and malignantepithelial ovarian cancer tissues,but presented with no significantdifference(F=1.588,P>0.05).3.The p-Akt protein was often detected in normal ovarian tissue, benign andmalignant epithelial ovarian cancer tissues.and the expression level presented withsignificant differences(F=17.525,P<0.05).p-Akt expression in epithelial ovariancancer group was higher than that in other histologic types(P<0.05).The expression level of p-Akt was related to the FIGO stage (P<0.05),but has no relation withpathologic types(P>0.05).4.There was a negative correlation between miR-145and p-Akt (r=-0.492,p=0.001).The expression between miR-145and Akt,Akt and p-Akt had nocorrelation(r=0.088,P=0.56;r=0.180,P=0.232).Conclusions：1.miR-145,Akt and p-Akt protein were expressed in normal ovarian tissue,benign and malignant epithelial ovarian cancer tissues.2.Low-expression of miR-145maybe associated with the tumorigenesis ofhuman epithelial ovarian cancer. High-expression of p-Akt might play certain roles inthe tumorigenesis and the FIGO stage of human epithelial ovarian cancer.3.Low-expression of miR-145and excessive activation of Akt have negativecorrelation in epithelial ovarian cancer.