Preliminary Study On Flavonoids Of Nervilia Fordii

Objective:Use Nervilia fordii as test object to study flavonoid antioxidant activity in vitro, in order to provide a reference of Nervilia fordii clinical new drug development, separation of compounds is supporting to look for new medicine source and to expand the medicine source; Methods:Based on preliminary studies, we use solvent extraction system to extract flavonoids from big-leaf Nervilia fordii; Secondly, comparing flavonoids differences of big-leaf, mid-leaf and small-leaf Nervilia fordii,which Nervilia fordii flavonoids extracted shows highest, will be selected to do reseach.Using UV visible spectrophotometry for the determination of total flavonoids. Then,pyrogallol autoxidation method, phenanthroline-Fe2+oxidation method, prussian blue method for the determination of total flavonoids in vitro antioxidant activity test. Finally, we studied flavonoid isolated exploration,in order to clear chemical composition.Results:Choose big-leaf Nervilia fordii to extract total flavonoids,we get high enriched total flavonoids fraction, it is eluted with50%polyamide,portion of89.25%, in Nervilia fordii herbs total flavonoids was0.023%. Furthermore, experiments show that flavonoids Nervilia fordii has significant antioxidant pharmacological activity. Rhamnocitrin was isolated.and we confirmed another flavonoid was rhamnazin.Conclusion:The significant antioxidant pharmacodynamic activity flavonoids from Nervilia fordii is easy to high-enriched get, so total flavonoids have antioxidant value of pharmaceutical formulation development. To provide a reference of Nervilia fordii clinical new drug development, and separation of compounds is supporting to look for new medicine source and expand the medicine source; In this study, the method of sodium nitrite-aluminum nitrate sodium hydroxide colorimetry as UV spectrophotometry was used to test total flavonoids of Nervilia fordii, experiments show that it passed methodological test.Study provied a reference and basis for testing total Flavonoids in Nervilia fordii.