| Objective: Osteoarthritis (OA) is a common chronic jointdisease. The main pathological characteristics is degeneration of articularcartilage. Finally lead to softening of the articular cartilage, fibrosis,fractureand damage of the articular surface,resulting in long-term pain anddysfunction.There’re many causes such as gender, age, obesity, occupationalactivity, injury, genetic and so on. OA diagnosed according to theirmanifestations,including imaging examination, and biochemical markerstests.In recent years,the biochemical markers tests developmet fast,whichplay an important role in the early dignosis of OA,lesions progress andevaluation of drug efficacy.The study found that keraten sulfate(KS), whichalmost only exists in the articular cartilage and disc cartilage aggrecan aminopolysaccharide, is accuracy and can be quantitative. As a direct marker, itcan be reflect the articular cartilage metabolism quite well. There are a lot ofmethods to treat OA,medication and non-medication are included.Acupuncture treatment, one of the most effective non-medication methods,has been widely recognized. Electro-acupuncture(EA) as a product of thecombination of traditional acupuncture and modern electricity,can improvethe effect of traditional acupuncture and is widely used clinically.Therefore,our study use the extensor Rabbit knee OA model to observe the expression of keratant sulfate in the rabbits’left knee osteoarthritis articular cartilageafter EA intervention,in aim of exploring the possible mechanism of EAtreatment.Methods:(1)interventions methods: choosed thirty6-month-oldhealthy New Zealand Rabbits as objects, then used statistical software togenerate random numbers to separate objects for normal control group,model control group and EA group. Each group included10rabbits. Thenimmobilized the rabbits’left knee in extention by plater cast. After6week,we first choosed the30th inch needles acupuncturing liangqiu, Zusanli,Xuehai,Yanglingquan,and then connected the SDZ-II electro-acupunctureinstrument to the needles, lastly we adjusted the treatment dose:4HZ sparewave,20HZ dense wave,0.5ms pulse width. treatment time:20min a day,10times continuously. At the same time, the other two groups were also fixedon the rabbit desk for the same equal time while the EA group was treated.After10days treatment,first make all the rabbits were killed by the airembolism method, secondly taked out the whole left knee joint and observedit visually, thirdly knifed a cartilage block from the left medial formalcondyle to the subcondral bone,which large to0.5cm×0.5cm×0.5cm.Afterfinish all those steps, we used10%neutral formalin solution to fixedarticular cartilages, then what we should do is to paraffin-embedded, sliced,and HE staining to observe all the index.(2)Outcome measures:①Evaluatedthe articular cartilage by using the Mankin’s score after observing itsmorphology visually.②Observed the cartilage surface smoothness, color, integrity and joint effusion by light microscope, then evaluated them usingthe Mankin’s score.③Immunohistochemical method:detected KS expressionin the knee cartilage by anti-rabbit KS reagents.(3)Statistical analysis: usedstatistical software SPSS17.0to analysis all the data by t-test and ANOVAseparately and then represent by x±s.Results:(1)cartilage morphology:thenormal control group rabbits:cartilage surface smoothly without fissure, thecolor was blue and white transparent without significant joint effusion.According to Mankin’s score were0.The model group rabbits: cartilagesurface was quite roughly with fissure, the color was pale yellow and filledwith joint effusion. EA group rabbits: cartilage surface relatively rough witha little joint effusion,the color displayed light yellow and the whole situationwas better than the model group.(2)HE-staining through the lightmicroscope:Normal control group rabbits: the articular cartilage structurewas clear with full tide line, cells arranged orderly, rounded cells bodies, andmatrix stained uniform.Model group rabbits: the articular cartilage structurewas coarse and the cell disordered arranged with cluster-like shape, the fulltide was disappeared and matrix stained shallow. EA group: the cartilagestructure was better than the model group, cells were arranged order than themodel group with full tide line. matrix stained more uniform than the modelgroup.Evaluated the three groups via Mankin’s score,and there was asignificant difference between the model groups and EA group(P<0.05).(3)KS expression detection in the knee articular cartilage: used Image Pro Plus software to evaluate the KS expression in the cartilage, then displayedthem by interated optical density (IOD) and analysis the data byANOVA.Compare the nomarl group to model group and EA groupseparately.And then The compared EA groupwith model group,there was asignificant difference among the three groups(P<0.05). Conclusion:(1)Immobilizing the rabbits’left knee in extention by plater cast can bettersimulate the human OA pathology progress and characteristic.(2)There exista certain correlations between the KS expression in the articular cartilageand the OA pathology progress. At the same time, KS can truly confirmed asa direct marker.(3)EA plays a important role in the knee osteoarthritistreatment. The mechanism may lies in the comprehensive effect between theacupuncture and electrophysiology.They together balance proteoglycansynthesis and decomposition.and thus improve cartilage metabolism,promote cartilage repair, delay the disease development. |
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