Silencing MACC1by RNA Interference Suppresses Proliferation And Migration Of Breast Cancer Cells MCF-7

Objective To explore the effect of metastasis associated in coloncancer1(MACC1) knockdown by RNA interference on the proliferationand migration and molecule mechanism in breast cancer cells.Methods The fluorescently-labeled siRNA-FAM targeting MACC1gene was chemically synthesized，and was then transfected into humanbreast cancer cell line MCF-7by siRNA Transfection Reagent. Inhibitionefficiency of MACC1mRNA and protein expression was examined byRT-PCR and Western blot. Moreover, MTT assay was applied to assess theinfluence of specific MACC1siRNA on cell growth．Transwell chambertest was used to observe the cancer cell migration ability. Furthermore,downstream migration-related targets of MACC1, S100A4and vimentin inMCF-7cell were investigated by Western blot.Results MCF-7cells showed significantly high expression ofMACC1．Transfection of specific MACC1siRNA-FAM into MCF-7cellsresulted in a highly-efficient declined expression of MACC181%atmRNA and75%at protein levels after48h，specific siRNA-FAM ofMACC1can suppress cell proliferation(p＜0.05) and decrease migrationability(p＜0.05) of MCF-7cells. We found knockdown of MACC1expression inhibit S100A4and vimentin protein expression in mcf-7cells. Conclusion Specific siRNA-FAM can silence MACC1efficiently andinhibit the proliferation and migration by regulating S100A4which is atranscriptional target of the Wnt/β-catenin-pathway in human breast cancercell MCF-7.