| Objective: the pathological changes of lung tissue in mice withPseudomonas aeruginosa pneumonia infections observed andcorresponding expression in lung tissue of calcium binding proteinS100A9RNA, and determine S100A9calcium binding protein contentand common inflammatory factor TNF-a in mouse blood, status and roleof calcium binding protein S100A9in mice induced by Pseudomonasaeruginosa lung infection in the. Methods: this experiment requires36mice, were randomly divided into control group, model group of123H12,6h12rats in model group, the three group. Through the ring capsularpuncture injection of Pseudomonas aeruginosa bacteria to establish themodel of pulmonary infection.36clean mice were randomly divided intoblank control group (12rats, referred to as the1group, ring capsuleinjection of saline, die molding process2), model group (24rats, byPseudomonas aeruginosa bacteria liquid, ring capsule injection ofequivalent2Maxwell unit concentrations were divided into3H and9htwo time. The modeling of death5), respectively, in3hours and9hoursof2time points were9and10respectively. The mice right lower lobelung tissues were observed with HE staining to observe pathological, blank control group, pathological changes in3H model group and9hmodel group; yield of S100A9mRNAPCR three in lung tissues of mice inthe reaction process using ordinary PCR detection system for real-timemonitoring, the final analysis of the experimental data, the difference andcompared between the three groups mice produced S100A9mRNA. Andsynchronization using double antibody sandwich enzyme linkedimmunosorbent assay (ELISA) method were used to determine the threeblood of mice in group S100A9and TNF-a content, S100A9calciumbinding protein differences in mouse blood and related department andvariation of TNF-a between the three groups. Results:(1) the pathologicalobservation of lung tissue in rats: right HE staining pathological results of200times under light microscope, normal control group (group1) andalveolar structure is more uniform and clear, there are a few lymphocytesand macrophages infiltration; model group (Group3H) mainly alveolarinflammation, alveolar in and around the visible exudate, neutrophils andlymphocytes, macrophage infiltration; model group (group9h) the mostsevere pulmonary alveolitis, in and around the visible exudation,infiltration of neutrophils and macrophages, lymphocytes, pathologicalinflammatory changes significantly heavier than the3H group.(2) PCRexperiment: the experiment to determine the level of template copynumber according to the electrophoresis bands of brightness, orexpression level. The experimental results show that the s100A9mRNA in the second,3group of the intensity of the bands intensity was higher ingroup1, suggesting that S100A9expression of model group wassignificantly higher than that of the control group, and after statisticalanalysis of3H and control group, the difference was statisticallysignificant (p<0.05), and9h compared with model group (P<0.05)meaningful differences.(3) S100A9calcium binding protein ELISAdetection: results showed that2, the content of S100A9in peripheralblood of3group was significantly higher than that in group1, and in3Hgroup increased most significantly,3h group compared with the controlgroup, the difference was statistically significant (p<0.05) compared with9h group (P<0.05) difference.(4) tumor necrosis factor TNF-aELISA testresults show that:2, the content of TNF-a in peripheral blood of3groupwas significantly higher than that of the1groups, the difference wasstatistically significant (p<0.05).(5) by combining the correlationanalysis of protein and tumor necrosis factor-a to S100A9calcium, thereis a positive correlation between the change trend of the model group,3Hr=0.77;9h model group, r=0.653.(1) conclusion: calcium binding proteinS100A9in mice of Pseudomonas aeruginosa pulmonary inflammatoryreaction.(2) the main protein in pneumonia appears early, the mechanismneeds further study. |
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