The Expression And Significance Of VEGF, EG-VEGF, TGF-β1in Polycystic Ovary

Objective: Polycystic ovary syndrome (PCOS) is a commondisorder affecting women of childbearing age whose main feature areoligo-ovulation or anovulation,hyperandrogenemia and Polycysticovaries，having various clinical manifestation. PCOS is associated withinsulin resistance,diabetes and obesity. The mechanisms of PCOS are notcompletely clear. Large numbers of immature follicle formation ofovulatory disorder infertility is an important cause of the disease, whichhas been found the relationship with the granule cell dysfunction. Inearly research we detected the expression of Survivin, XIAP andCaspase-3in polycystic ovarian granulosa cells and their relationship,discussing its role of the gene of apoptosis inhibition protein andCaspase-3in polycystic ovary development, which provides someexperimental basis for the treatment of polycystic ovarian syndrome.Because of the complex etiology, PCOS exists various pathogenesisThis research we hope study the pathogenesis of this disease from thevascular growth factor aspect. Prominent features of PCOS pathologyare high vascular hyperplasia of ovarian interstitial and follicle,resulting in abnormal ovulation. The disorder regulation of angiogenicfactors may cause vascular dysplasia. The disorder regulation of angiogenic factors may cause vascular dysplasia, Angiogenic factors arealso located in ovary, which regulate ovarian granulosa cells andtheca cell through autocrine and paracrine and intracellular regulatorypathways,playing an important role in the development of normalfollicles. Abnormal disturbance regulation of ovary factors maycontribute to the cause of anovulation in polycystic ovary. VEGF,EG-VEGF,TGF-β1are both angiogenesis factor and the pathogenesisof ovarian local regulating factors, this study detected the expression ofVEGF, EG-VEGF and TGF-β1in polycystic ovary to get furtherinvestigation of the mechanism of PCOS. Methods:1. Choose theexperimental group and control group which met inclusion and exclusioncriteria of the ovary paraffin embedded tissue. The experimental groupof26patients, aged14-39years old, average28.8±6.65years;12casesin the control group, between the age17-39year old, average29.5±5.72years old. The patients had no history of hormone administration, vaginalbleeding history.2.Ovary paraffin embedded tissue machine serialsections at room temperature,after the spreading of attaching them tothe slide, put in60℃constant temperature oven for about24hours.Immunohistochemical Envision method was used to detect the expressionlevel of VEGF, EG-VEGF and TGF-β1in the groups of ovarianfollicular granulosa cells, theca cells and ovarian stromal.3. Under themicroscope observation: analysis of the results were carried out byAx-70microscopic imaging system in OLYMPUS company of Japan, each section randomly was selected8(×200) high power field. Positivecells: cell structure is clear, composition of cell membrane, cytoplasm andnucleus of the complete structure; the positive particles were located,good stability; cell staining with background color obviously. Thepositive expressions of VEGF in vascular endothelial cells weredetermined by the patina appearing brown or yellow particles, nobrown or yellow for the negative. The positive expressions of EG-VEGFin vascular endothelial cells were determined by the patina appearingbrown or yellow particles, no brown or yellow for the negative. TGF-β1expressions were determined by the cytoplasmic in yellow brown, yellowor yellow particles, no brown or yellow for the negative.4.Imageacquisition: images acquisition from computer,by the10×10magnification, positive expression of the follicles and interstitial, and findthe graafian follicle; each layer of the graafian follicle cells wereobserved by10×10times and×200magnification.Photograph everyfollicle cell layer with8consecutive field.5.Image analysis: using IPP(Image Pro plus)6image analysis software, analysis theimmunohistochemical staining of cystic follicular granulosa cells, thecacells and ovarian interstitial and calculation of optical density quantitativevalue (OD), the average optical density(Average Optical Density; AOD)of immunohistochemical staining is optical density values of the slice.The data obtained from the different groups were compared and thedifference between the two groups mean±standard deviation (±),was analyzed by SPSS13statistical software, while the differences betweengroups were analyzed by t test, P <0.05was significant difference.Results: Compared with the age of two groups, P>0.05, no significantdifference. In PCO group VEGF high expressed in ovarian granulosacells, EG-VEGF high expressed in ovarian endometrial layer andmatrix, TGF-β1in antral follicle thecal cells and ovarian stromal highexpressed. The expression of VEGF in PCO group was significantlyhigher than that in normal group, P <0.05, there was statisticalsignificance. Expression of EG-VEGF in PCO group was higher than thatof the normal group P <0.05, there was statistical significance.Theexpression of TGF-β1in PCO group was the same as in normalovarian group, P>0.05， no statistical significance. VEGF wasnegatively correlated with EG-VEGF in the PCO group. TGF-β1andVEGF had no correlation with EG-VEGF in the PCO group.Conclusion: VEGF and EG-VEGF had a certain relationship with theoccurrence and development of PCO. The incidence of TGF-β1may hadno obvious relationship with PCO. In PCO group VEGF was negativelycorrelated with EG-VEGF, which may explain the two distributions oftime and space in the pathogenesis of PCO was not the same.