Metabolome Analysis Of Oligodendroglia Cells Infected With Herpes Simplex Virus Ytype1by1H NMR*

Background:Herpes simplex virus type1(HSV-1) is an enveloped, double-stranded DNA virus, which has a high infection rate and a wide infection range, mainly causes herpes simplex encephalitis (HSE) in the central nervous system. HSE is a major threatening human health diseases and paid close attention for the higher incidence. In addition, there are closed relationship between HSV-1and Alzheimer’s disease, intractable focal epilepsy, multiple sclerosis or acute disseminated encephalomyelitis. But little is known about the molecular mechanisms underlying infection with HS V-1. Previous studies have shown that HS V-1infection disrupts the cellular metabolism, but the lack of an overall research.Objective:To investigate the effects of herpes simplex virus type1(HSV-1) on a human oligodendroglia cells (OL cells) metabolism and explore the molecular mechanisms underlying infection with HSV-1. Methods:OL cells were ramdomly divided into two groups:one group was infected with HSV-1, the other group was not, extracted the metabolites in the culture medium respectively, and analyzed the metabolites by the proton nuclear magnetic resonance (1H NMR) spectra and partial least square discriminant analysis (PLS-DA).Results:There were significant differences in the metabolic profiles between the two groups. The biomolecules observed to be obvious different include amino acids, lactate, creatine and glucose.Conclusion:HSV-1markedly disrupts OL cells metabolic homeostasis, may affects the citric acid cycle, energy metabolism and purine nucleotide biosynthesis, and institutes its own specific metabolic program.