| Our country has rich edible fungus resources and the edible mushroom use in our country’s history has been for thousands of years. Polysaccharide is one of the active ingredients of the fungus, with enhanced immunity, anti-tumor, anti-oxidation, and other biological activity, so the research of fungi polysaccharides has important practical significance. This article selects two kinds of rare and precious edible fungus, Copyinds Comatus and pholiota nameko as materials, conducted a series of in vitro activity of its polysaccharide components:1、The polysaccharide from Coprinus comatus is isolated by taken different extraction methods:using hot water extraction to obtain water extraction polysaccharide named WCP, ultrasonic assisted extraction is used to prepare UCP, through quaternary ammonium precipitation method for the purification and separation,UCP is divided into UCP-2and UCP-3.Polysaccharides from Coprinus comatus isolated by hot water (WPC) are carboxymethylated and sulfated to obtained carboxymethylated Coprinus comatus polysaccharide(CWCP) and sulfated Coprinus comatus polysaccharide(SWPC). The infrared spectra scanning of these five Coprinus polysaccharide characterize the structure of the functional groups. The analyse of anti-tumor activity of polysaccharide WCP, UCP-2, UCP-3, SWCP and CWCP on human leukemia K562cells in vitro is carried out by MTT assay. The affect of in vitro anti-tumor activity of coprinus comatus polysaccharides extracted through different processes is investigated. The results showed that three kinds of Coprinus comatus polysaccharides have Cytotoxicity effect on K562cells. The ultrasound is likely to cause the connection pattern change of monosaccharides thus resulting in change of bioactivity. The inhibition effect of SWCP and CWCP are obvious, sulfation and carboxymethylation modifications help to improve the biological activity.2、Pholiota nameko is an important plant fungus with edible an medicinal value, in order to study the in viro biological activity of water-extracted crude polysaccharide of Pholiota nameko, in this article, reduction ability was researched, antioxidant activity was studied by DPPH method and lipid peroxidation induced by Fe2+method, antitumor activity was studied by MTT assay and Placenta blue cell count detection. Experimental results showed that PNP had a DPPH free radical scavenging capability close to Vc in high concentration. PNP had inhibitory actions on lipid peroxidation, and with the increasing of the concentration, the inhibitory effect increased, but the general trend is not big. MTT assay showed that PNP inhibited the growth of K562cells in vitro, exhibited dose-dependent and time-based effect, the highest inhibition ratio is35.03%when treated with PNP in800μg/mL for48h. In addition, the result of cell cycle analysis by flow cytometer showed that PNP could block the K562cell in G1phase.3、Using chemical modification method for sulfated polysaccharide modification structure(SPNP), the infrared spectra (IRS) of the sulfation polysaccharide is measured, sulfation polysaccharide has a sulfuric acid base-O-SO3-the S=O stretching vibration characteristic absorption peak at1235.32cm-1, the analyse of anti-tumor activity of sulfated polysaccharide on human leukemia K562cells in vitro is carried out by MTT assay, the results show that SPNP has a more siginificant antitumor activity compared with water extraction polysaccharide, its inhibitory activity increased before they are weakened with the increase of polysaccharide concentrations and action time. |
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