| Objective: To detect the expressions of GMⅡ (Golgi α-mannosidaseⅡ), E-cadherin and α-catenin in ovarian epithelial tumor tissues andovarian tumor cells with the ability of difference metastasis and thecorrelation.Methods: The expressions of GMⅡ, E-cadherin and α-catenin proteinsin ovarian epithelial malignant tumor tissues from46cases (includingomentum metastasis or lymph node metastatasis from27cases), ovarianepithelial borderline tumor tissues from15cases and ovarian epithelialbenign tumor tissues from12cases were detected byimmunohistochemistry. The expression levels of GMⅡ, E-cadherin andα-catenin mRNAs and proteins in A2780, SKOV-3and HO-8910with theability of differenence metastasis were detected by RT-PCR,immunofluorescence and Western blotting, respectively.Results: The positive rate of GM Ⅱ (87%) in ovarian epithelialmalignant tumor tissues were significantly higher than those in ovarianepithelial borderline tumor tissues and epithelial benign tumor tissues; the normal expression rates of E-cadherin, α-catenin (20%and28%) inovarian epithelial malignant tumor tissues were significantly lower thanthose in ovarian epithelial borderline tumor tissues (80%and60%) andepithelial benign tumor tissues (100%and83%); The positive rate of GMⅡwere higher in tumor tissues with omentum metastasis or lymph nodemetastasis than those in tumor tissues without omentum metastasis orlymph node metastasis, the normal expression rates of E-cadherin,α-catenin were lower in tumor tissues with omentum metastasis or lymphnode metastasis than those in tumor tissues without omentum metastasis orlymph node metastasis (P﹤0.05), GMⅡ and E-cadherin, α-catenin arenegatively correlated. The fluorescence density of GMⅡ was graduallyincreased in A2780, SKOV-3and HO-8910cells, whereas the fluorescencedensity of E-cadherin and α-catenin was gradually decreased. Theexpression levels of GMⅡ mRNAs and proteins was gradually increasedin A2780, SKOV-3and HO-8910cells, whereas the expression levels ofE-cadherin and α-catenin mRNAs and proteins was gradually decreased,and the difference was significantly among three cell lines (P﹤0.05).Conclusion: GMⅡ, E-cadherin, α-catenin may play an important rolein malignant progression of ovarian tumor, and GMⅡ may be a marker ofprognosis and a therapeutic target for ovarian tumor. |
