Study On The Role Of Sulfydral Redox Agent In The Control Of Insulin Secretion And Its Mechanism

Objective: To explore the role of sulfydral redox agent in the control of INS-1cellsinsulin secretion and its possible mechanisms.Methods:1. Insulin secretion was evaluated in INS-1pancreatic beta cells by astandard insulin radioimmunoassay. Briefly, the cells at approximately70%confluence were trypsinized. The resultant cell suspension was seeded into24-wellcell culture plates. The cells were maintained at37℃in a humidified5%CO2incubator.2. The role of DTDP, DTBNP and DTT in the control of glucose stimulated insulinsecretion in INS-1cells was explored.3. The pretreated cells were also used to examine potassium depolarization-evokedinsulin secretion in the presence of DTDP, DTBNP and DTT.4. The effect of DTDP and DTBNP on the regulation of glucose stimulated insulinsecretion in the presence of L-type CaVchannel and IP3receptor blocker nifedipine orKATPchannel blocker diazoxide was also investigated.5. In the same time, the experiment of DTDP and DTBNP controlling KCl stimulatedinsulin secretion under the condition of L-type CaVchannel and IP3receptor blockernifedipine or KATPchannel blocker diazoxide was carried out.Results:1. DTDP and DTBNP both can increase glucose stimulated insulin secretionin INS-1cells, which can be eliminated by DTT.2. In the INS-1cells insulin secretion experiment, DTDP and DTBNP can bothincrease KCl stimulated insulin secretion. And they can both be eliminated by DTT.3. For nifedipine pretreated INS-1cells insulin secretion experiment, DTDP and DTBNP both can increase glucose stimulated insulin secretion. But the insulinsecretion increase rate is lower than that of the non-nifedipine group.4. In diazoxide pretreated INS-1cells insulin secretion experiment, DTDP andDTBNP can both increase glucose stimulated insulin secretion. But the insulinsecretion increase rate is lower than the group without diazoxide treatment.5. For nifedipine pretreated INS-1cells insulin secretion experiment, DTDP andDTBNP both can increase KCl stimulated insulin secretion. But the insulin secretionincrease rate is lower than that of the non-nifedipine group.6. In diazoxide pretreated INS-1cells insulin secretion experiment, DTDP andDTBNP can both increase KCl stimulated insulin secretion. But the insulin secretionincrease rate is lower than the group without diazoxide treatment.Conclusion:1. Sulfydral redox agent can regulate INS-1cells insulin secretion.2. The mechanisms of sulfydral redox agent in the control of INS-1cells insulinsecretion may be related to the regulation of the activity of KATPchannel, L-type Cavchannel and IP3receptor.