Research Progress In The Correlation Between ERCC1、BRCA1and MicroRNA Gene In Non-small Cell Lung Cancer

ERCC1、BRCA1were considered a keeper of the genome, in a variety of tumorsimportant signaling pathways play a role of tumor suppressor, The most useful choiceor not was through the ERCC1、BRCA1genes detection were to guide the choice ofplatinum-based In non-small cell lung cancer (non-small cell lung cancer NSCLC).But for which don’t tissue samples obtained by surgery patients, ERCC1、BRCA1genesdetection becomes a problem, The study found that MicroRNA in development anddrug resistance of tumor are also plays a very important role, whether there is acorrelation between ERCC1、 BRCA1and MicroRNA in the process of cancerdevelopment in what relations in NSCLC, and related research rarely reported. Dueto time issues and the latest research shows that:ERCC1and BRCA1are of the samein guiding significance in tumor drug We according to a report in the genome and therelated literature, and finally determine the MicroRNA-146-a/b,182,155asBRCA1mRNA and correlation between MicroRNA research object and usingreal-time polymerase chain reaction (PCR) to detect BRCA1mRNA and MicroRNA-146-a/b,182,155in NSCLC tissue adjacent to carcinoma and expression level,Analysis BRCA1mRNA and MicroRNA-146-a/b,-182-155, the relationshipbetween for the subsequent research and the genetic diagnosis, treatment andprognosis of NSCLC forecast as early as possible to find new targets to provide somebasis.Collected in January2011-January2013in lianyungang city bengbu medicalcollege affiliated second hospital of the new hospital patients with untreated paraffinembedding pathological specimens from30cases (surgical specimens, and the-Ⅳstage I lung cancer diagnosed pathology remarkably, all patients without tumor associated treatment), the male24cases, female6cases, aged33-88(60.67+9.76)years old,14cases of adenocarcinoma and squamous cell carcinomas in16cases. Ⅰ-Ⅱ phase of the23cases, Ⅲ-Ⅳ7cases;13patients with lymph node metastasiswithout lymph node metastasis,17cases. Using real-time rt-pcr detection of30casesⅠ-Ⅳ stage non-small cell lung cancer tissue adjacent to carcinoma andBRCA1mRNA and the expression level of micrornas in the specimen.The expressionof BRCA1mRNA relative amounts in the tissue adjacent to carcinoma was (7.790+5.109)/(10.1316.534mm) in30cases of lung cancer tissue samples, MicroRNA-146-a/b,182,155in carcinoma and the relative amounts to express quantity respectively(42.667+34.293)/(6.378+/-6.351),(40.96+35.89/9.25+/-6.60),(9.48+9.34/23.29+/-23.19),(17.85+/13.86), had statistical significance (P <0.05),BRCA1mRNA and MicroRNA-146-a/b in NSCLC tissue adjacent to carcinomaand expressing the trend on the expression of MicroRNA-155and the consistent andhad statistical significance (P <0.05), while BRCA1mRNA and MicroRNA-182hasno statistical significance (P>0.05).Expression of MicroRNA-146-a/b,-182-155were related to pathogenesis of non-small cell lung cancer, and can be considered asthe independent early diagnosis factor, and the MicroRNA-146-a/b,155genesmainly involved in tumor may regulate the expression of BRCA1mRNA through therelated process.