The Effect Of The Hexavalent Chromium-Polluted Groundwater On The Blood Glucose And Livers Of The Mice As Well As The Antagonistic Action Of Vtamin C

ObjectiveTo explore the effect of the hexavalent chromium (Cr(Ⅵ)) pollutedgroundwater on the liver and blood glucose (Glu) of the mice and theinterference of vitamin C (Vit C).MethodsA total of80healthy and clean male Kunming mice of6-8weeks wererandomly divided into8groups: the control group (distilled water), the chromiumpolluted well water group with12.35mg/L (Cr(Ⅵ))which was collected fromsomewhere polluted, potassium dichromate alone groups with differentconcentration of34.91(12.35mg/L Cr(Ⅵ)),349.1,698.2,1396.4mg/L andcombined action groups with Vit C(100mg/ml)+698.2and1396.4mg/Lpotassium dichromate, ten rats in each group. All groups were exposed by oralgavages. The potassium dichromate groups and the groundwater group wereexposed at a dose of25ml/kg. Vit C groups were exposed at a dose of5ml/kgfirstly, and then exposed with potassium dichromate half an hour later. Theexposure lasted for30consecutive days. The poisoning manifestations wereobserved in the course of the experiment. After exposure.To collect blood andliver tissue. The Automatic Biochemistry Analyzer detected the levels of ALT and AST in serum. Liver tissue superoxide dismutase (SOD) activity andglutathione(GSH), malondialdehyde(MDA) content were analyzed byspectrophotometer; The change of pathological were observed by HE stainingmethod.ResultsIn the test, obvious symptoms of poisoning were observed in thegroundwater group and groups treated with different concentration of potassiumdichromate alone. Compared with control group, the levels of AST and ALTactivity as well as MDA in liver tissue were higher and the levels of SOD activityin liver, GSH as well as Glu were lower in the groundwater group and groupstreated with different concentration of potassium dichromate alone.The differences were statistical significance (P <0.05). The levels of AST, ALTactivity, Glu, SOD activity in liver, GSH and MDA were no significant change inthe Vit C pretreated groups. As the concentration of potassium dichromateincreased, the levels of AST, ALT activity and liver MDA content were on the rise;while the levels of SOD activity in liver and GSH were on the decrease.Compared with same concentration of potassium dichromate alone groups, thelevels of AST, ALT activity and liver MDA content in Vit C pretreated groups werelower and the levels of Glu, SOD activity in liver and GSH were higher.The differences were statistical significance (P <0.05). Compared with34.91mg/L potassium dichromate alone group，the levels of AST, ALT activity and liverMDA content in the groundwater group were higher and the levels of Glu, SODactivity in liver and GSH are lower. The differences were statistical significance(P <0.05).ConclusionThe Cr(Ⅵ)polluted groundwater can cause liver damage in mice and other heavy metal pollution may exist. Vit C has antagonism on the liverdamagecaused by potassium dichromate.