Investigation Of Antitumor And Related Bioactive Compounds Produced By A Fungus From Shark’s Gill

As cancer is one of the most fatal threats to human beings, the screening of anticancer drugs has become the focus of drug screening. Due to their species diversity, marine microorganisms can produce diversified active substances with various structures. Therefore, as a new source of anti-cancer drugs, marine microorganisms have become the focus of marine drug research for their great development potential.In this paper, we firstly didperformed hydrostatic liquid fermentation in different medium for24fungal strains of Active fungus separated isolated from sShark gill department Their extracts’ bioactivities were evaluated by DNA damage repair experiment, anti-brine shrimp (Artemia parthenogenetica L.) larvae and antitumor activity test. Those potent extracts were futtherly analyzed by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC). In the above comprehensive screening, three strains’ extracts, namely BP2-BP2T1, AP2-AP2T1, and BG2-TBG2-2, exhibitedthey are strong resistance to brine shrimplarvicidal, antibacterial and antitumor activity as well as their distinct secondary metabolite fingerprints. The bioactive constituents in these three extracts were tracked and investigated on their structural types by color development reactions, whose results showed their possibility as they are different with each others in main composition. Active ingredients of tracking and chemical colour reaction were discussed with3strains fungus. We Preliminary speculated that they may contain indole derivatives, other alkaloids, phenols, and terpenoidsalkaloids of active ingredients.After HPLC-bioactivity test-TLC analysis towards the three strains’active extracts, strain TBG2-2was selected as an ideal producer strain for its rich metabolites and strong bioactivity. Its fermentation extract on BG2medium showed selective inhibitory activity against the DNA repair-defective E. coli strain and100%lethal ratio towards brine shrimp larvae. For further investigation on chemical constituents, TBG2-2was statically fermented in20liter’s BG2broth. Under the guidance of larvicidal and anti-E. coli tests, ten active fractions from its crude solvent extracts were separated and purified by repeated use of column chromatography on (CC) on silica gel,RP-18, and Sephadex LH-20, as well as by semi-preparative HPLC. As the result, six compounds were obtained. Among them, three compounds were structurally elucidated to be known antitumor compounds, fructigenine A, viridicatol, and cyclopenol by spectroscopic methods (mainly using nuclear magnetic resonance (NMR) and mass spectra (MS)) and by comparison with references’ data. These bioactive compounds showed their value for further research and development. Nevetheless, the novel bioactive compounds in this strain are still to be investigated in further study.The present study showed the potential of shark gill-derive fungi’s secondary metabolites in the development of antitumor drugs, antibiotics, and pesticides.