The Effects Of H102with Stem Cell To Apoptosis In The Brain Of AD Mice

Objective:By observing the combined effects of H102and hUCMSC to APP transgenic mice behavior and their brain cells’ apoptosis, study hUCMSC and H102treatment in Alzheimer’s disease and the possible mechanism. Methods:1. Umbilical cord mesenchymal stem cells have been isolated, cultured, tested, and transplanted. Take the second generation of umbilical cord mesenchymal stem cell line Dil fluorescent marker.2. The AD mice were randomly divided into model group, hUCMSC2weeks group, the H102group, hUCMSC4weeks group, H102with hUCMSC group and a group of C57BL/6J mice with the same age and background was set as normal. Model group and normal control group were treated with normal saline solution, while the H102group was treated with H102every day. stem cell2weeks group and stem cell4weeks group were injected with stem cell.Test the memory of rember by Morris Water Maze (MWM), Immunohistochemical stain and Western bloting were done with Bad, Bax, Bcl-2, Bcl-xl.Results:1. Umbilical cord mesenchymal stem cells in the training process get a lot of amplification, cell markers was about90%, and distribution Uniformly.2. The function of Umbilical cord mesenchymal stem cells and H102to APP transgenic mouse animal models.2.1The Morris water maze test:2.1.1Place Navigation Training:hUCMSC2week escape latency of mice slightly shorter than the model group, but the difference was not significant (P>0.05), and longer than the normal group (P<0.01);hUCMSC4week escape latency in mice reduced the model group (P<0.01), but still higher than normal extension (P<0.01). H102group, H102+hUCMSC escape latency of mice compared with model group reduced (P<0.01), H102+hUCMSC group compared with H102group escape latency shorter (P>0.05).2.1.2Spatial Probe Test:hUCMSC2week compared with the model group, slightly extension(P>0.05), compared with the normal is still shorter (P<0.01); hUCMSC4week, compared with the model group, was longer residence time (P<0.01), but still shorter than the normal group (P<0.01); H102group, H102+hUCMSC group time and the third quadrant model group was longer compared to the differences were significant (P<0.01), H102+hUCMSC group compared with H102, but the difference was not significant (P>0.05).2.2the effect of hUCMSC and H102on the APP transgenic mouse brain cell apoptosis:Bad, Bax immunohistochemical staining:hUCMSC4week group content of less than model group (P<0.01), still more content than the normal group(P<0.01); HI02group, H102+hUCMSC group, the cortex and hippocampus CA3area compared with model group, positive cells decreased (P<0.01), H102+hUCMSC group compared with the H102group, positive cells slightly decreased (P>0.05).Bcl-2, Bcl-xl Immunohistochemistry:hUCMSC4weeks group content than the model group increased (P<0.01), still less than normal levels (P<0.01); H102injection group, H102+hUCMSC group, the cortex and positive cells in hippocampal CA3area was increased compared with model group (P<0.01), H102+hUCMSC group compared with the H102, positive cells increased, but the difference was not significant (P>0.05).Western blot detection:H102injection group, H102+hUCMSC group Bad, Bax protein content compared with the control group (P<0.01), Model mice brain Bcl-2protein content than normal mice decreased (P<0.01). H102group, H102+hUCMSC Bcl-2levels in mice than in model group increased (P<0.01), compared with the normal group (P>0.05).Conclusions:H102is a kind of Promising drugs; the Method of hUCMSC transplantation is need to be improved.