Transcatheter Arterial Injection Of SPIO-NS/Doxorubicin/PVA Treatment Of Rabbit VX2Liver Tumor Efficacy Study

OBJECTIVE:In order to compare the effect of chemoembolization effect of SPIO-NS/doxorubicin/PV and conventional iodinol in treating rabbit VX2liver tumor.METHODS:60New Zealand rabbit with tumor were randomized into6groups, which were SPIO only group (A group), conventional iodatol+pirarubicin group (B group), PVA+pirarubicin group (C group), pirarubicin group (D group), SPIO-NS/doxorubicin/PV group (E group), and NS control group (F group). CT perfusion examination was performed on7and14days post procedure and tumor volume and growth rate were calculated as to compare tumor size and growth condition. MRI examination was performed as to observe SPIO distribution within tumor tissue and the contrast effect on tumor imaging comparing to conventional MRI contrast agent.5rabbits were sacrificed on day14post procedure and histopathological examination (Prussian blue staining, HE staining, VEGF and CD34staining) and drug concentration within tumor were performed to compare the treatment outcome difference among these groups. The remaining5rabbits were kept alive as to compare the survival rate among these groups.RESULT:The tumor size and growth rate were significantly lower than the other five groups. MRI examination demonstrated SPIO could be used for tumor tracing. In histopathologic examinations, Prussian blue staining showed SPIO existence and distribution and A group was found to be most effective which demonstrated massive concrete necrosis and tumor cell fragments under light microscope and homogeneous non-structure material under electric microscope. Tumor proliferation index Ki-67in A group was detected to be significantly lower than the other groups (P<0.05). Intra-and peri-tumor drug concentration was significantly higher than the other5groups, while in normal hepatic tissue significantly lower(P<0.05). Total incidences of toxic effects and side effects within14days post procedure was significantly lower than the other5groups, as well as vomiting and loss of appetite incidence (P<0.05).Hair removal the same group F with the incidence of adverse events, and other groups have differences. The survival time was28.1±4.5d、23.7±5.0d、18.3±8.0d、22.6±5.2d,26.0±2.5d、23±4.5d respectively for A, B, C, D, E, and F group, while A group was significantly longer.CONCLUSION:SPIO only group (A group) was superior to other treatment protocol (B-F group), with obvious embolisation and chemotherapy effect, ability of tumor-tracing on MRI, mild toxic effect, longer survival time, and a presumably intermittent-releasing manner. B-E group had positive effect in treatment of rabbit VX2tumor, and F group had no treatment at all.