The Role Of Galectin-1on The Injure Of Human Podocyte Cells Induced By High Glucose

ObjectiveTo investigate the expression of Galectin-1and podocyte injury marker podocin induce by high glucose on HPCs and the relationship between Galectin-1and podocyte injury.Transfect Galectin-1siRNA in HPCs to inhibit Galectin-1expression, detect podocin expression, investigate whether Galectin-1can inhibit high glucose-induced podocyte injury.MethodsHPCs were randomly divided into five groups:normal control group,25mM high glucose treatment HPCs12h,24h, and48h groups,25mM MN intervene HPCs48h group. Real-time PCR, Western blot and immunofluorescence were used to analyze the expression of Galectin-1and podocin.Use TransIT-TKO-Transfection Reagent transfected Galectin-1siRNA to HPCs, HPCs were randomly divided into three groups:normal control group (untransfected HPC), high glucose group (untransfected HPC+25mM high glucose) and transfected group (transfected Galectin-1siRNA in HPC+25mM high glucose), Real-time PCR and Western blot were used to analyze the mRNA and protein expression of Galectin-1, podocin.Results(1) Under normal culture conditions, HPC express podocin, only express few Galectin-1.(2) Treatment of HPCs with25mM high glucose different time resulted in a time-dependent increased in the mRNA expression of Galectin-1,12h,24h,48h Galectin-1mRNA expression compared with control group were1.3times,2.2times(P<0.05) and2.5times(P<0.05); the protein expression of Galectin-1also in a time-dependent increased,12h,24h,48h Galectin-1protein expression compared with control group were1.25times,1.87times and2.19times, has statistically significant (P<0.05); the25mM MN intervene HPCs48h group has not statistically significant (P>0.05) compare with control group.(3) Treatment of HPCs with25mM high glucose different time resulted in a time-dependent decreased in the mRNA expression of podocin,12h,24h,48h podocin mRNA expression compared with control group were60%,44%,33%, has statistically significant (P<0.05); the protein expression of podocin also in a time-dependent decreased,12h,24h,48h podocin protein expression compared with control group were71%,32%,24%, has statistically significant (P<0.05); the25mM MN intervene HPCs48h group has not statistically significant (P>0.05) compare with control group.(4) Immunofluorescence results show that Galectin-1was mainly expressed in the podocyte cytoplasm and nucleus, and the podocin expressed on the cell membrane; Treatment of HPCs with25mM high glucose different time resulted in a time-dependent increased the Galectin-1fluorescence expression compared with the control group, decreased the podocin fluorescence expression.(5) After transfect Galectin-1siRNA to HPCs, compared with high glucose group, Galectin-1mRNA and protein expression were inhibited (P<0.05), while podocin expression was markly up-regulated, has significant differences (P<0.05).Conclusion(1) Galectin-1was expressed in HPCs, and high glucose can upregulate the expression of Galectin-1in a time dependent manner in HPCs.(2) High glucose can induced HPCs podocin expression.(3) Galectin-1related to podocyte injury induced by high glucose.(4) Galectin-1siRNAmay suppress high glucose induced HPCs injury.