The Significance Of KIM-1、CC16in Acute Kidney Injury And Acute Lung Injure

Objective:To investigate the change of KIM-1and CC16in the patients with AKI and ALI, and study the significance of acute kidney/lung injury.Methods:A study was conducted in25pure AKI patients,15pure ALI patients,25AKI and ALI patients, and23non-smoking controls (age, race, and gender-matched). To collect clinical presentations, urinary and plasma samples. These samples were centrifuged at2000r/min for10minutes, and the supernatant were stored at-20℃until assay. ELISA method to detect urinary KIM-1、plasma KIM-1、urinary CC16and plasma CC16; colorimetric method to detect urinary N-acetyl-beta-D-glucosaminidase (NAG).Results:1. Compared with non-smoking control group, the urinary NAG、 urinary KIM-1、plasma KIM-1and plasma CC16values were significantly increased in AKI group (P<0.05); the urinary CC16and plasma CC16values were significantly increased in ALI group (P<0.05); the urinary NAG、urinary KIM-1、plasma KIM-1、urinary CC16and plasma CC16values were significantly increased in AKI and ALI group (P<0.05).2. Correlation analysis showed:In AKI patients, urinary KIM-1was positive correlated with urinary NAG (r=0.493, P<0.01), while the plasma KIM-1was no correlated with urinary NAG. In ALI patients, urinary CC16was negative correlated with oxygenation index, the same as plasma CC16(P<0.01). In AKI and ALI group, urinary KIM-1、 plasma KIM-lwere positive associated with urinary CC16and plasma CC16respectively (P<0.05).3. Receiver operator characteristic curve (ROC) analysis showed: In AKI, the area under the curve (AUC) of urinary KIM-1was0.781(95%CI0.688～0.875, P<0.01); plasma KIM-1was0.988(95%CI 0.000～1.000, P<0.01); urinary NAG was0.798(95%CI0.708～0.888, P<0.01). In ALI, the AUC of urinary CC16was1.000(95%CI1.000～1.000, P<0.01); plasma CC16was0.849(95%CI0.764～0.935, P<0.01).Conclusion:1. Urinary NAG, urinary KIM-1and plasma KIM-1were obviously increased in AKI, showed that these indicators can be used to early diagnose AKI.2. Urinary CC16and plasma CC16were significantly elevated in ALI, and with high sensitivity, suggested that CC16can serve as a good biomarker for ALI.3. Urinary and plasma KIM-1and CC16were significantly increased in acute kidney/lung injury and with good correlation, showed that KIM-1, CC16have clinical significance for diagnosis and prognosis of acute kidney/lung injury.