Fibroblast Growth Factor21Levels In Non-obese Diabetic Mice

Objective:FGF21is a member of the FGF family, which has been recently identified as the master hormonal regulator of glucose, lipids and overall energy balance. In this study, our aim is to investigate the serum and pancreatic levels of FGF21in NOD/ShiLtJ mice.Methods:13female NOD/ShiLtJ mice and13age-matched female BALB/c mice as healthy control were used in our study. The NOD/ShiLtJ mice and BALB/c mice were both randomly divided into fasting group (fasted for16hours) and postprandial group (fed normally). We detected the blood glucose of the NOD/ShiLtJ mice weekly. Once the blood glucose of a NOD/ShiLtJ mouse was higher than16.7mmol/L twice, it was diagnosed as diabetes. Diabetic NOD/ShiLtJ mice and age-matched BALB/c mice were executed, and their serum and pancreas were collected for use. The concentration of serum and pancreatic FGF21were detected by ELISA. The expression of pancreatic FGF21and insulin were examined by immunofluorescent staining.Results:(1) The postprandial level of serum FGF21in NOD/ShiLtJ mice was significantly lower than which in the control (P<0.05);(2) The fasting and postprandial pancreatic levels of FGF21in NOD/ShiLtJ mice were both significantly lower than which in the control (P<0.05);(3) The distribution of FGF21and insulin in mice pancreas were almostly located in the same site.(4) FGF21was expressed in MIN6cell line.Conclusions:(1) The postprandial level of serum FGF21in NOD/ShiLtJ mice were significantly lower than which in the healthy mice;(2) The pancreatic levels of FGF21in NOD/ShiLtJ mice were significantly lower than which in the healthy mice.