The Study Of The Aire Gene Expression On Myasthenia Gravis With Thymoma

The application of immunohistochemistry (IHC) and realtime fluorescence quantitative polymerase chain reaction (FQ PCR) were used to detect AIRE protein and its gene expression in myasthenia gravis with thymoma tissue and surrounding thymus tissues. The following issues were investigated:1) clinical features of myasthenia gravis (MG) with thymoma patients;2) the pathological features of MG related thymoma;3) the relationship between the pathological features of thymoma and the AIRE protein and gene expression.In the research, patients’ clinical data treated in the MG treatment centers from2008to2012were collected in the People’s Liberation Army No.309Hospital. Besides,93cases of thymoma biopsy specimens in Pathology from2008to2012were collected. AIRE protein and gene expression were detected by SP immunohistochemistry and realtime fluorescence quantitative PCR.The clinical features of MG with thymoma were summarized as the following. It was discovered by pathological study of MG patients that the average age of onset of thymoma group was significantly higher than that of non-thymoma group. After the age of30, the incidence of thymoma group was significantly increased. The MG clinical types were mainly Ⅲ and Ⅳ the multi (P=0.00) in the thymoma group. Type B2was the most common in the MG patients with thymoma. However there’s no obvious difference between different thymoma in the gender proportion, age of onset, initial symptoms and clinical genotyping. The histologic classification of thymoma was associated with Masaoka stage (P<0.05) other than gender, age, tumor size or lymph follicles. The degree of capsule invasion and fatty infiltration were different in the different types of thymoma. The capsule invasion degree was gradually increased by the order of A-AB-B1-B2-B3. The necrosis degree varies according to different types of thymoma, in which B2, B3were the most common. There’s no statistical difference in pathological change of thymus tissue surrounded by various thymoma. AIRE gene expression rate of thymoma was lower than that of thymoma tumor around tissue. The former was27.8%while the latter was45.8%, with a significant difference (P=0.042). The AIRE gene expression of thymoma tissue was irrelevant to the patient’s gender, age, MG clinical classification, pathological type, tumor size, or the capsule invasion (P>0.05). AIRE-positive cells in the normal thymus tissue were mainly distributed in medullary thymic; whereas AIRE-positive cells were rarely distributed in thymoma tissue regardless of the different types of thymoma.We could draw the following conclusion from experiments. The thymoma group’s average age of onset was significantly higher than that of non-thymoma group. After the age of30, the incidence of thymoma group increased significantly. The histologic classification of thymoma was associated with Masaoka stage (P<0.05). The capsule invasion degree was gradually increased by the order of A-AB-B1-B2-B3. AIRE gene expression rate of thymoma was lower than that of thymoma tumor around tissue. AIRE-positive cells in the normal thymus tissue were mainly distributed in medullary thymic; whereas AIRE-positive cells were rarely distributed in thymoma tissue regardless of the different types of thymoma.