| Objective:We analy the expression of eIF3a protein in ovarian cancer cell lines through western blotting. Overexpression and suppression of elF3a were carried out to determine the effect of altered elF3a expression on cellular response to platinum and the expression of XPC protein. The study is to test the hypothesis that elF3a affect cellular response to platinum-based chemotherapy.Methods:MTT assay was used to analysis the cell cellular response to cisplatin of A2780and A2780/DDP cell lines. We analy the expression of eIF3a protein in these two ovarian cancer cells through western blotting. Ectopic overexpression and RNA interference suppression of elF3a were carried out in A2780/DDP and A2780cell lines, respectively, to determine the effect of altered elF3a expression on cellular response to cisplatin by using MTT assay. Western blotting analyses were carried out to determine the expression of eIF3a and XPC protein by using cells with altered elF3a expression.Results:1) Western blot results revealed that the eIF3a protein expression levels were significantly higher in A2780cell line than A2780/DDP cell line.2) elF3a expression associated with response of ovarian cancer patients to platinum-based chemotherapy. MTT results revealed that A2780cell line had lower platinum resistance than A2780/DDP cell line.3) elF3a suppression and overexpression, respectively, increased and decreased the cellular resistance to platinum and the expression of DNA repair proteins. Conclusions:1) elF3a plays an important role in regulating the expression of DNA repair proteins which, in turn, contributes to cellular response to platinum-based chemotherapy.2) eIF3a might be as a potential biomarker predicting chemothera-peutic drugs responses. |
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